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Concentration of controls in dilution pools for expanded range experiments |
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| Pool |
Conc. A Dil. 2 |
Conc. B Dil. 2 |
Conc. C Dil. 2 |
Conc. D Dil. 2 |
Conc. A Dil. 3 |
Conc. B Dil. 3 |
Conc. C Dil. 3 |
Conc. D Dil. 3 |
Conc. A Dil. 4 |
Conc. B Dil. 4 |
Conc. C Dil. 4 |
Conc. D Dil. 4 |
|
|
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| Pool 7 |
250 |
2 |
10 |
50 |
500 |
4 |
20 |
100 |
5,000 |
40 |
200 |
1,000 |
| Pool 8 |
50 |
250 |
2 |
10 |
100 |
500 |
4 |
20 |
1,000 |
5,000 |
40 |
200 |
| Pool 9 |
10 |
50 |
250 |
2 |
20 |
100 |
500 |
4 |
200 |
1,000 |
5,000 |
40 |
| Pool 10 |
2 |
10 |
50 |
250 |
4 |
20 |
100 |
500 |
40 |
200 |
1,000 |
5,000 |
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Concentration is given as mass ratios, so that "250" represents 1:250,000 or 1 ng of RNA transcript per 250 ng of background RNA, where the spike amount is adjusted for its length. Concentrations of the initial stock, "Dilution 1" pools are shown in Table 4. | ||||||||||||
External RNA Controls Consortium BMC Genomics 2005 6:150 doi:10.1186/1471-2164-6-150 |
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