Figure 4.

Phylogram of CAMK ePKs. Kinase domains from the TriTryp predicted proteins classified as CAMKs by BLAST or CAMK-like from the T. brucei tree in Figure 2 were analyzed using MRBAYES. Also included are CAMKs from all families present in humans (Hs), plus several S. cerevisiae (Sc) and a P. falciparum (Pf) kinase. Nodes with a bootstrap value greater than 0.95 are marked by a dot, while values ranging from 0.7–0.95 are indicated numerically. Similar results were obtained with PAUP*. Kinase domains are indicated by systematic gene IDs, which are abbreviated in the case of L. major to Lm in lieu of LmjF. Additionally, the invariant digits in the T. cruzi systematic names were deleted (all gene names start with Tc00.1047053). Only one T. cruzi allele was included for each gene. ψ marks a T. cruzi and T. brucei gene that are predicted to be non-functional due to a lack of a recognizable subdomain 1. The CAMK-like kinases classified as unique by BLAST are marked, as are the trypanosomatid CAMKs with EF-hand accessory domains. ScCDC28, a CMGC kinase, was used as an outgroup.