Table 5 |
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|
Primers used to amplify short fragments of the Squilla mantis genome. Majority/minority strand are with reference to the ancestral pancrustacean sequence. These primers will work for almost all arthropods and for many other metazoans as well. Reference "JB" is J. Boore pers. comm. Reference "CEC" refers to primers designed for this project. |
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|
Gene |
Majority strand |
Sequence |
Minority strand |
Sequence |
Reference |
|
|
|||||
|
COX1 |
Lco1490 |
GGTCAACAAATCATAAAGATATTGG |
Hco2198 |
TAAACTTCAGGGTGACCAAAAAATCA |
38 |
|
COX1-COX2 |
CO1DL |
CCWCGWCGWTAYTCWGAYTAYCCWGA |
CO2DL |
CWGAATARRCATAWSWTCARTATCATTG |
JB |
|
ATP6-COX3 |
ATP6.AANMM |
GCCGTACGGCTTGCAGCNAAYATRAT |
CO3DL2 |
ACWACGTCKACGAAGTGTCARTATCA |
CEC, JB |
|
COX3 |
CO3DL1 |
TGGTGGCGAGATGTKKTNCGNGA |
CO3DL2 |
ACWACGTCKACGAAGTGTCARTATCA |
JB |
|
ND5 |
ND5-R-DL |
TARAAKCCWGMTARAAAWGGKAWWCC |
ND5-F-DL |
TWYTATTAGGKTGAGATGGKYTNGG |
JB |
|
ND4 |
ND4-R_DL |
GARGAWCAKAWWCCRTGAGCAATYAT |
ND4-F-DL |
CCKAARGCYCAYGTKGARGCYCC |
JB |
|
CYTB |
Cytb424-449 |
GGWTAYGTWYTWCCWTGRGGWCARAT |
Cytb876-847 |
GCRTAWGCRAAWARRAARTAYCAYTCWGG |
JB |
|
ND1 |
ND1DL1 |
CCTTCWGCAAAATCGAAAGGGGYHCG |
ND1.YIQIR |
AAGATCCTTGGATAYATYCARATYCG |
JB, CEC |
|
ssrRNA |
12Sb-3' |
GAGGGTGACGGGCGGTGTGT |
12sa-5' |
AAACTGGGATTAGATACCCTATTAT |
JB |
|
lsrRNA |
16SBRH |
CCGGTCTGAACTCAGATCACGT |
16SARL |
CGCCTGTTTATCAAAAACAT |
39 |
|
|
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|
Cook BMC Genomics 2005 6:105 doi:10.1186/1471-2164-6-105 |
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