Table 1

RT-PCR validation results for tissue and disease-specific splice isoforms. The experiments are categorized into three groups viz. tissue specific isoforms predicted via ESTs related to non-normalized libraries (1 to 4), tissue specific isoforms predicted only via ESTs derived from normalized libraries (5 to 16) and disease-specific isoforms (17 to 20). For some of the variants represented by normalized libraries, standard PCR did not reveal the isoforms. However, five of these isoforms were detected using refined PCR conditions. The experiments frequently validated the isoforms and the tissue type, but the predicted specificity was rarely verified.
Isoform	Gene	Chr.	Unigene	EST Evidence	ESTs	Cycles	Isoform	Specificity	Comment (Most sensitive PCR)	Norm. Level

1	Unknown	11	Hs.112250	testis	3	39	+	+
2	ISGF3G	14	Hs.1706	stomach	10	39	+	-	Ubiquitous
3	MRPL42	12	Hs.112110	stomach-lymph	5	39	+	-	Ubiquitous
4	SGN3	17	Hs.6076	testis	3	39	-	?
5	PC326	13	Hs.279882	testis	9	39	+	+	testis [36]	Rot-5
6	LMO7	13	Hs.5978	brain	5	39	+	-	brain, testis, eye(?) [18]
7	HRD1	8	Hs.274122	brain	3	39	+	-	brain, eye, thymus, salivary gland, kidney
8	Unknown	1	Hs.24119	pancreas	4	39	+	-	approx. 10 tissues	Cot-20
9	BCLG	12	Hs.11962	testis	4	39,78	?,+	+,+		Cot-5
10	RBPMS	8	Hs.80248	placenta	4	39,78	?,+	-,-	Ubiquitous
11	SCML1	X	Hs.109655	testis	12	39,78	?,+	+,-	approx. 6 tissues	Rot-5
12	WNK1	12	Hs.432900	kidney	3	39,78	?,+	+,-	Digestive system [28]	Cot-25
13	NY-CO-10	5	Hs.23557	testis	3	39,78	-,+	?,+		Cot-5
14	Unknown	11	Hs.169100	testis	3	39,78	-,-	?,?		Rot-5
15	Unknown	16	Hs.48396	breast	4	39,78	-,-	?,?		Cot-230
16	CIDE-A	18	Hs.249129	breast	4	39,78	-,-	?,?		Cot-230
17	KCNAB2	1	Hs.298184	tumor	29	39	+	-	Ubiquitous
18	SNRP70	19	Hs.174051	stomach ascites	25/26	39	+	-	Ubiquitous
19	RAB1	14	Hs.227327	tumor	39/95	39	+	-	fetal(kidney, thymus, liver, spleen), ovary [19]
20	Unknown	7	Hs.272688	tumor	12	39,78	-,-	?,?	relevant tumor sample not in set

Gupta et al. BMC Genomics 2004 5:72 doi:10.1186/1471-2164-5-72