 Methodology articleStrengths and weaknesses of EST-based prediction of tissue-specific alternative splicingShobhit Gupta1 1 Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestr. 73, D-14195 Berlin – Germany 2 German Resource Center for Genome Research, INF 580, 69120 Heidelberg – Germany
BMC Genomics 2004, 5:72doi:10.1186/1471-2164-5-72
Additional filesAdditional File 1: List of tissues for which tissue specific transcripts are predicted. This is a text file with a listing of all tissues for which specific trascripts exist along with the number of ESTs related to individual tissues. Furthermore, the ESTs derived from normalized libraries and the specific variants predicted via such ESTs are also listed. Format: TXT Size: 1KB Download file Additional File 3: Detailed description of RT-PCR experiments This is an excel file containing the primer sequences used for RT-PCR experiments along with detailed comments on the gel pictures subsequently obtained. Format: XLS Size: 22KB Download file This file can be viewed with: Microsoft Excel Viewer Additional File 2: RT-PCR picture (jpeg file) showing the expression pattern of gene PRAME This gene shows specific expression for several tumor types, along with testis as the only normal tissue. Format: JPEG Size: 174KB Download file |
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