Table 9

Presumptive human chromosome 17 loci that were mapped in Ambystoma

Marker ID

Primersa

Diagnosisb

LGc

Symbold

RefSeq IDe

E-valuef


Pl_6_E/F_6

F-GAAAACCTGCTCAGCATTAGTGT

ASA

ul

PFN1

NP_005013

E-34

R-TCTATTACCATAGCATTAATTGGCAG

Pl_5_G/H_5

F-CTATTTCATCTGAGTACCGTTGAATG

PE (A)

23

CGI-125

NP_057144

E-56

R-TAATGTAGAACTAAATGGCATCCTTC

E-CCATGGTGCAGGAAGAGAGCCTATAT

Pl_0.4_A/B_1

F-GTCTCATTATCCGCAAACCTGT

SP

1

RPL19

NP_000972

E-67

R-ATTCTCATCCTCCTCATCCACGAC

Pl_4_B_7/8

F-CCTAGAACATTACCAAAACAGACTCA

RD (Dpn II)

1

KRT10

NP_061889

E-17

R-AATGAAGAAGTATTGCATGTGAGAAC

Pl_4_B_9/10

F-GAACTTGTTGGCAGGTTTCTCTT

RD (AciI)

1

KRT17

NP_000413

E-146

R-CTAGTGATAGGTTGGACATACCAGAG

Pl_10_C/D_4

F-CTCCACTATTTAAAGGACATGCTACA

PE (A)

1

SUI1

NP_005792

E-48

R-TTAATATAGCACAACATTGCCTCATT

E-TGCTACATTAATGTAATAAACGGCATCATC

Pl_6_E/F_11

F-AAGAGAAGTTCCTAGATGAGTTGGAG

PE (A)

1

HSPC009

NP_054738

E-26

R-TGAAGAGAGAACTCAAAGTGTCTGAT

E-TCATGTTTTGCTCTGCTGTGCAGT

Pl_9_A/B_10

F-TGATAGTTTCTGGATTAAGACGAGTG

PE (T)

1

FLJ13855

NP_075567

E-15

R-CTTAGAGCCATTGTTACAAGATGTTC

E-GTGATCTAGTGGGATCAAACCCTAAAGACC

Pl_10_C/D_9

F-AAAGTGCCAAGAAGGAGATTAACTT

PE (T)

9

NME1

NP_000260

E-71

R-GAGCTCAGAAAACAAGGCAGTAAC

E-AAATGGATCTACGAGTAGACCTTGACCC

Pl_9_C/D_9

F-GAGTCTCCTTTAGGATTGACGTATCT

PE (T)

23

FLJ20345

NP_060247

E-17

R-GCTATGTGAGCAGAGATAAAAGTCAG

E-GTTACAGCATCAGTGGGATGTGGTATGT

Pl_8_C/D_9

F-AGGATACCAACCTCTGTGCTATACAT

PE (C)

15

H3F3B

NP_005315

E-66

R-TAAATGTATTTACAAACCGAAAGCAA

E-CGTGGCGAGCGTGCCTAGT

Pl_9_C/D_4

F-GTGGTTATTTGTAACATTTCGTTGAC

PE (A)

8

SFRS2

NP_003007

E-40

R-AATTACATTTGGGCTTCTCAATTTAC

E-TTTTTAAACGCGTAAAAATGTTAACAGA

Pl_6_C/D_5

F-CCGTAAATGTTTCTAAATGACAGTTG

PE (G)

2

ACTG1

NP_001605

0

R-GGAAAGAAAGTACAATCAAGTCCTTC

E-GATTGAAAACTGGAACCGAAAGAAGATAAA


aSequences are 5' amplification primers, 3' amplification primers, or primer extension probes, and are preceded by F-, R-, and E- respectively. bGenotyping methods are abbreviated: allele specific amplification (ASA), size polymorphism (SP), restriction digestion (RD), primer extension (PE). Diagnostic restriction enzymes and diagnostic extension bases are provided in parentheses. cAmbystoma linkage group ID. "ul" designates markers that are unlinked. dOfficial gene symbols as defined by the Human Genome Organization Gene Nomenclature Committee http://www.gene.ucl.ac.uk/nomenclature/ webcite. eBest BLASTX hit (highest e-value) from the human RefSeq database using the contig from which each marker was designed as a query sequence. fHighest E-value statistic obtained by searching contigs, from which EST markers were designed, against the human RefSeq database.

Putta et al. BMC Genomics 2004 5:54   doi:10.1186/1471-2164-5-54

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