Table 5

Comparison of microarray and QRT-PCR estimates of FC for selected genes

Gene

Est. Array FC* IVT

Est. Array FC dIVT

QRT-PCR Lower 90%

QRT-PCR Est. FC

QRT-PCR Upper 90%

TNF

1/19.53

1/153.58

1/99.52

1/11.31

1/1.29

K6A

1/16.2

1/17.95

1/344.62

1/56.69

1/9.33

SFP

47.7

54

1/2.02

1/1.05

1.82

IGFB

10.82

8.8

1.42

2.51

4.41

LOH

1/21.21

1/5.66

1/11.28

1/3.61

1/1.15

AXL

36.82

13.02

1/1.21

2.51

7.57

DMP

1/18.94

1/29.04

1/55.24

1/28.34

1/14.54

GR

1/4.67

1/20.49

1/50.05

1/10.37

1/2.15

EGFR

4.58

6.27

1/37.44

1/10.85

1/3.15

NFKB

2.38

5.66

10.08

12.98

16.71

*Est. Array FC in bold met criteria P(FC < 1/2 ∪ FC > 2) >.99.

Gold et al. BMC Genomics 2004 5:2   doi:10.1186/1471-2164-5-2