Research article
Identification of Bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization
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* Corresponding author: Vito G DelVecchio vdelvecchio@vitalprobes.com
1 Loyola Hall of Science, University of Scranton, Scranton, Pennsylvania 18510, USA
2 Institute of Molecular Biology and Medicine, University of Scranton, Scranton, Pennsylvania 18510, USA
3 Present address: Schott Glass Technologies Inc. 400 York Avenue, Duryea, PA 18642, USA
4 Present address: Vital Probes, Inc., 1300 Old Plank Road, Mayfield, PA 18433, USA
BMC Genomics 2004, 5:15 doi:10.1186/1471-2164-5-15
Published: 12 February 2004Additional files
Additional File 1:
Table 2. Survey genomic DNA blot results. Indication of detection (+) or no detection (-) by each 32 P-labeled insert of the 52 SSH-selected clones used to probe survey Southern blots containing samples of HindIII-digested genomic DNAs of B. anthracis 7700, B. anthracis AC1, B. anthracis RA3, B. anthracis 7611, B. thuringiensis 97-27, Bacillus Ba813+ S8553/2, Bacillus Ba813+ III-BL, B. cereus F17289, B. cereus 14579T, B. thuringiensis CT43, B. thuringiensis huazhongensis, and B. thuringiensis 10792T.
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Table 3. Annotation of the 29 B. anthracis-specific SSH clones. For each of the 29 B. anthracis-specific SSH clones reported in this paper this table provides their B. anthracis A2012 chromosome coordinates, overlapping ORF designations, closest homolog genes with putative function, e value, accesssion number and comparative results with other recently published B. anthracis SSH study [32].
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