Table 4

Effect of column cleanup on the fidelity and yield of amplification.a,b

Protocol

G-50 cleanup

w/o G-50 cleanup


Number of amplifications

7

5

Correlation coefficient

Virtual

0.83

0.85

Average

0.79 ± 0.03

0.81 ± 0.02

Yield of aRNA (μg)

11.9 ± 2.8

15.9 ± 2.7

Fold of amplification

397 ± 93

530 ± 90


aData were obtained from comparing expression level of 12,305 clones using the described selection criteria. bInput BC2 total RNA is 3 μg and amplification was done without TS primer.

Zhao et al. BMC Genomics 2002 3:31   doi:10.1186/1471-2164-3-31

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