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Effect of column cleanup on the fidelity and yield of amplification.a,b |
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| Protocol |
G-50 cleanup |
w/o G-50 cleanup |
|
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|
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| Number of amplifications |
7 |
5 |
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| Correlation coefficient |
Virtual |
0.83 |
0.85 |
| Average |
0.79 ± 0.03 |
0.81 ± 0.02 |
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| Yield of aRNA (μg) |
11.9 ± 2.8 |
15.9 ± 2.7 |
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| Fold of amplification |
397 ± 93 |
530 ± 90 |
|
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aData were obtained from comparing expression level of 12,305 clones using the described selection criteria. bInput BC2 total RNA is 3 μg and amplification was done without TS primer. | |||
Zhao et al. BMC Genomics 2002 3:31 doi:10.1186/1471-2164-3-31 |
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