Table 3

Effect of DNA ligase on the fidelity of amplification.a,b

Protocol

Affymetrix

Affymetrix w/o ligase


Number of amplifications

6

5

Correlation coefficient

Virtual

0.84

0.86

Average

0.79 ± 0.04

0.82 ± 0.04

Yield of aRNA (μg)

24.1 ± 4.7

19.2 ± 5.9

Fold of amplification

803 ± 157

640 ± 197


aData were obtained from comparing expression level of 13,783 clones using the described selection criteria. bInput BC2 total RNA is 3 μg.

Zhao et al. BMC Genomics 2002 3:31   doi:10.1186/1471-2164-3-31

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