Table 1

Correlation coefficients of amplified and unamplified expression levels of 14,044 genes selected according to the described criteria. Amplifications with or without TS primer and with two different ds cDNA cleanup protocols were performed on BC91 total RNA.

Column for ds cDNA cleanup

Bio-6

G-50


Reference RNA amplified

Total RNA

Poly(A)+ RNA

Total RNA

Poly(A)+ RNA


w/o TS

Virtual

0.84 (n = 2)

0.77 (n = 4)

0.82 (n = 2)

0.80 (n = 4)

Average

0.83 ± 0.01 (n = 2)

0.73 ± 0.07 (n = 4)

0.81 ± 0.00 (n = 2)

0.76 ± 0.05 (n = 4)

TS

Virtual

0.81 (n = 2)

0.79 (n = 3)

0.77 (n = 1)

0.71 (n = 2)

Average

0.80 ± 0.05 (n = 2)

0.74 ± 0.04 (n = 3)

0.68 ± 0.02 (n = 2)


Zhao et al. BMC Genomics 2002 3:31   doi:10.1186/1471-2164-3-31

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