Figure 4.

Detection of purified circles with ERCA. A 10-fold serial dilution of preformed circles ranging from 10⁶ to about 1 copy was used as substrate in an ERCA reaction. (a) Real-time profile of the amplification signal obtained with each of the circle dilutions. (b) A standard curve of threshold cycle (C_T) vs. circle number shows that the reaction is linear over six logs of the target.