Detection of purified circles with ERCA. A 10-fold serial dilution of preformed circles ranging from 106 to about 1 copy was used as substrate in an ERCA reaction. (a) Real-time profile of the amplification signal obtained with each of the circle dilutions. (b) A standard curve of threshold cycle (CT) vs. circle number shows that the reaction is linear over six logs of the target.
Faruqi et al. BMC Genomics 2001 2:4 doi:10.1186/1471-2164-2-4