Open Access Highly Accessed Research article

Integrating transcriptional, metabolomic, and physiological responses to drought stress and recovery in switchgrass (Panicum virgatum L.)

Eli Meyer12*, Michael J Aspinwall23, David B Lowry2, Juan Diego Palacio-Mejía2, Tierney L Logan2, Philip A Fay4 and Thomas E Juenger2

Author Affiliations

1 Department of Integrative Biology, Oregon State University, Cordley Hall 3029, Corvallis, OR 97331, USA

2 Department of Integrative Biology, University of Texas at Austin, 1 University Station C0930, Austin, TX 78712, USA

3 Hawkesbury Institute for the Environment, University of Western Sydney, Penrith, NSW 2751, Australia

4 USDA-ARS Grassland Soil and Water Research Laboratory, Temple, TX 76502, USA

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BMC Genomics 2014, 15:527  doi:10.1186/1471-2164-15-527

Published: 26 June 2014

Abstract

Background

In light of the changes in precipitation and soil water availability expected with climate change, understanding the mechanisms underlying plant responses to water deficit is essential. Toward that end we have conducted an integrative analysis of responses to drought stress in the perennial C4 grass and biofuel crop, Panicum virgatum (switchgrass). Responses to soil drying and re-watering were measured at transcriptional, physiological, and metabolomic levels. To assess the interaction of soil moisture with diel light: dark cycles, we profiled gene expression in drought and control treatments under pre-dawn and mid-day conditions.

Results

Soil drying resulted in reduced leaf water potential, gas exchange, and chlorophyll fluorescence along with differential expression of a large fraction of the transcriptome (37%). Many transcripts responded differently depending on time of day (e.g. up-regulation pre-dawn and down-regulation mid-day). Genes associated with C4 photosynthesis were down-regulated during drought, while C4 metabolic intermediates accumulated. Rapid changes in gene expression were observed during recovery from drought, along with increased water use efficiency and chlorophyll fluorescence.

Conclusions

Our findings demonstrate that drought responsive gene expression depends strongly on time of day and that gene expression is extensively modified during the first few hours of drought recovery. Analysis of covariation in gene expression, metabolite abundance, and physiology among plants revealed non-linear relationships that suggest critical thresholds in drought stress responses. Future studies may benefit from evaluating these thresholds among diverse accessions of switchgrass and other C4 grasses.

Keywords:
Drought; Recovery; Switchgrass; Panicum virgatum; Gene expression; RNA-seq