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Open Access Research article

Regulatory and coding genome regions are enriched for trait associated variants in dairy and beef cattle

Lambros Koufariotis123*, Yi-Ping Phoebe Chen1, Sunduimijid Bolormaa2 and Ben J Hayes123

Author Affiliations

1 Faculty of Science, Technology and Engineering, La Trobe University, Melbourne, Victoria 3086, Australia

2 Department of Environment and Primary Industries, AgriBio Building, 5 Ring Road, Bundoora, Victoria 3086, Australia

3 Dairy Futures Co-operative Research Centre, 5 Ring Road, Bundoora, Victoria 3086, Australia

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BMC Genomics 2014, 15:436  doi:10.1186/1471-2164-15-436

Published: 6 June 2014

Abstract

Background

In livestock, as in humans, the number of genetic variants that can be tested for association with complex quantitative traits, or used in genomic predictions, is increasing exponentially as whole genome sequencing becomes more common. The power to identify variants associated with traits, particularly those of small effects, could be increased if certain regions of the genome were known a priori to be enriched for associations. Here, we investigate whether twelve genomic annotation classes were enriched or depleted for significant associations in genome wide association studies for complex traits in beef and dairy cattle. We also describe a variance component approach to determine the proportion of genetic variance captured by each annotation class.

Results

P-values from large GWAS using 700K SNP in both dairy and beef cattle were available for 11 and 10 traits respectively. We found significant enrichment for trait associated variants (SNP significant in the GWAS) in the missense class along with regions 5 kilobases upstream and downstream of coding genes. We found that the non-coding conserved regions (across mammals) were not enriched for trait associated variants. The results from the enrichment or depletion analysis were not in complete agreement with the results from variance component analysis, where the missense and synonymous classes gave the greatest increase in variance explained, while the upstream and downstream classes showed a more modest increase in the variance explained.

Conclusion

Our results indicate that functional annotations could assist in prioritization of variants to a subset more likely to be associated with complex traits; including missense variants, and upstream and downstream regions. The differences in two sets of results (GWAS enrichment depletion versus variance component approaches) might be explained by the fact that the variance component approach has greater power to capture the cumulative effect of mutations of small effect, while the enrichment or depletion approach only captures the variants that are significant in GWAS, which is restricted to a limited number of common variants of moderate effects.

Keywords:
Variants component analysis; Regulatory genome; GWAS prioritization; Enrichment depletion