Table 1

List of 31S. sclerotiorum effector candidates selected based on their annotation
SPEPs containing PFAM domains found in fungal effectors
Protein ID Annotation PFAM domain(s) Length Comments
SS1G_00642 Chitin binding protein Chitin recognition protein (PF00187.14) 563
SS1G_02014 Chitin binding protein Chitin binding domain (PF03067) 426
SS1G_04786 Chitin binding protein Chitin recognition protein (PF00187.14) 399
SS1G_09392 Starch binding domain containing protein Chitin binding domain (PF03067) 398
SS1G_12336 Chitin binding protein Chitin binding domain (PF03067) 294
SS1G_14184 Agglutinin isolectin 3-like Chitin recognition protein (PF00187.14) 245
SS1G_12509 LysM domain protein LysM (PF01476) 447
SS1G_07836 Concanavalin A lectin glucanase Peptidase_A4 (PF01828) 252
SS1G_08698 Ricin-type toxin Ricin-type beta-trefoil lectin domain (PF00652) 409
SS1G_01593 Serine protease inhibitor Peptidase inhibitor I9 (PF05922) 95
SS1G_03282 Serine protease inhibitor Peptidase inhibitor I9 (PF05922) 522
SS1G_12605 Alkaline serine protease alp1 Peptidase inhibitor I9 (PF05922) 400
SS1G_03611 Cystein-rich protein CFEM (PF05730) 119
SS1G_13935 Cystein-rich protein CFEM (PF05730) 529
SPEPs containing nuclear localization signal (NLS)
Protein ID Annotation PFAM domain(s) Length Comments
SS1G_01866 Ring-7 protein PHD-like zinc-binding domain (PF13771); Ring finger domain (PF13639) 425 NLS (118–141)
SS1G_03146 - - 193 NLS (147–155)
SS1G_04309 - - 177 NLS (82–87)
SS1G_05895 Zinc finger CCCH-type domain containing protein Zinc finger C-x8-C-x5-C-x3-H type (PF00642) 310 NLS (256–291)
SS1G_05938 - - 212 NLS (143–150)
SS1G_06787 - - 430 NLS (189–250; 340–349)
SS1G_06890 - - 284 NLS (66–120; 143–167; 225–245)
SS1G_07404 - DUF3108 (PF11306) 282 NLS (65–79; 118–138; 223–233)
SS1G_09050 - - 454 NLS (410–413)
SS1G_11108 Ribosomal protein s17 - 373 NLS (338–348)
SS1G_13142 - - 131 NLS (89–99)
SPEPs showing homology to fungal effectors or fungal effector candidates
Protein ID Annotation PFAM domain(s) Length Comments
SS1G_03080 SsNEP1 Necrosis inducing protein (PF05630) 246 Homolog to B. cinerea NEP1 (Staats et al., 2007); P. Sojae NIP (Qutob et al., 2002); studied in (Bashi et al., 2011)
SS1G_11912 SsNEP2 Necrosis inducing protein (PF05630) 245 Homolog to B. cinerea NEP1 (Staats et al., 2007); P. Sojae NIP (Qutob et al., 2002); studied in (Bashi et al., 2011)
SS1G_00849 AltA-1 allergen analog - 152 Homolog to C. hingginsianum HE651255_CHEC91 Kleemann et al., 2012)
SS1G_02904 CVNH protein CyanoVirin-N Homology domain (PF08881) 169 Homolog to C. hingginsianum HE651243_CHEC80 Kleemann et al., 2012)
SS1G_08858 Deuterolysin metalloprotease Deuterolysin metalloprotease (M35) family (PF02102) 354 Homolog to M. oryzae AvrPita (MGG_15370 - Orbach et al., 2000)
SS1G_10096 Cerato-platanin (PF07249) 137 Homolog to B. cinerea BcSpl1 (Frias et al., 2011); H. atroviridis EPL1 (Seidl et al., 2006); M. oryzae MSP1 Jeong et al., 2007); C. hinginsianum HE651160_CHEC5 Kleemann et al., 2012)

Results from Blast2GO automated annotation, PFAM domain searches and BlastP against known fungal effectors were considered. Table entries are ordered as in main text. SPEP, S. sclerotiorum secreted protein induced in planta.

Guyon et al.

Guyon et al. BMC Genomics 2014 15:336   doi:10.1186/1471-2164-15-336

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