Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Research article

Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors

Ana M Bocsanczy1, Ute CM Achenbach2, Arianna Mangravita-Novo3, Marjorie Chow4 and David J Norman1*

Author Affiliations

1 Department of Plant Pathology, University of Florida, IFAS, Mid-Florida Research and Education Center, 2725 Binion Rd., Apopka, FL 32703, USA

2 Development Lead North-East Europe, Syngenta Agro GmbH, Am Technologiepark 1-5 63477, Maintal, Germany

3 Burnham Institute for Medical Research at Lake Nona, 6400 Sanger Road, Orlando, FL 32827, USA

4 ICBR Proteomics Core, University of Florida, Gainesville, FL 32610, USA

For all author emails, please log on.

BMC Genomics 2014, 15:280  doi:10.1186/1471-2164-15-280

Published: 12 April 2014

Abstract

Background

Ralstonia solanacearum, the causal agent of bacterial wilt, is a genetically diverse bacterial plant pathogen present in tropical and subtropical regions of the world that infects more than 200 plant species, including economically important solanaceous crops. Most strains of R. solanacearum are only pathogenic at temperatures between 25 to 30°C with strains that can cause disease below 20°C considered a threat to agriculture in temperate areas. Identifying key molecular factors that distinguish strains virulent at cold temperatures from ones that are not is needed to develop effective management tools for this pathogen. We compared protein profiles of two strains virulent at low temperature and two strains not virulent at low temperature when incubated in the rhizosphere of tomato seedlings at 30 and 18°C using quantitative 2D DIGE gel methods. Spot intensities were quantified and compared, and differentially expressed proteins were sequenced and identified by mass spectrometry (MS/MS).

Results

Four hundred and eighteen (418) differentially expressed protein spots sequenced produced 101 unique proteins. The identified proteins were classified in the Gene Ontology biological processes categories of metabolism, cell processes, stress response, transport, secretion, motility, and virulence. Identified virulence factors included catalase (KatE), exoglucanase A (ChbA), drug efflux pump, and twitching motility porin (PilQ). Other proteins identified included two components of a putative type VI secretion system. We confirmed differential expression of 13 candidate genes using real time PCR techniques. Global regulators HrpB and HrpG also had temperature dependent expression when quantified by real time PCR.

Conclusions

The putative involvement of the identified proteins in virulence at low temperature is discussed. The discovery of a functional type VI secretion system provides a new potential virulence mechanism to explore. The global regulators HrpG and HrpB, and the protein expression profiles identified suggest that virulence at low temperatures can be partially explained by differences in regulation of virulence factors present in all the strains.

Keywords:
Bacterial wilt; Temperature; Virulent strains at low temperature; Type VI secretion system; Stress response