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Open Access Research article

Sequence, distribution and chromosomal context of class I and class II pilin genes of Neisseria meningitidis identified in whole genome sequences

Mirka E Wörmann1, Corey L Horien2, Julia S Bennett3, Keith A Jolley3, Martin C J Maiden3, Christoph M Tang1, Ellen L Aho2* and Rachel M Exley1*

Author Affiliations

1 Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK

2 Department of Biology, Concordia College, Moorhead, MN, USA

3 Department of Zoology, University of Oxford, Oxford OX1 3PS, UK

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BMC Genomics 2014, 15:253  doi:10.1186/1471-2164-15-253

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2164/15/253


Received:29 November 2013
Accepted:25 March 2014
Published:1 April 2014

© 2014 Wörmann et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Abstract

Background

Neisseria meningitidis expresses type four pili (Tfp) which are important for colonisation and virulence. Tfp have been considered as one of the most variable structures on the bacterial surface due to high frequency gene conversion, resulting in amino acid sequence variation of the major pilin subunit (PilE). Meningococci express either a class I or a class II pilE gene and recent work has indicated that class II pilins do not undergo antigenic variation, as class II pilE genes encode conserved pilin subunits. The purpose of this work was to use whole genome sequences to further investigate the frequency and variability of the class II pilE genes in meningococcal isolate collections.

Results

We analysed over 600 publically available whole genome sequences of N. meningitidis isolates to determine the sequence and genomic organization of pilE. We confirmed that meningococcal strains belonging to a limited number of clonal complexes (ccs, namely cc1, cc5, cc8, cc11 and cc174) harbour a class II pilE gene which is conserved in terms of sequence and chromosomal context. We also identified pilS cassettes in all isolates with class II pilE, however, our analysis indicates that these do not serve as donor sequences for pilE/pilS recombination. Furthermore, our work reveals that the class II pilE locus lacks the DNA sequence motifs that enable (G4) or enhance (Sma/Cla repeat) pilin antigenic variation. Finally, through analysis of pilin genes in commensal Neisseria species we found that meningococcal class II pilE genes are closely related to pilE from Neisseria lactamica and Neisseria polysaccharea, suggesting horizontal transfer among these species.

Conclusions

Class II pilins can be defined by their amino acid sequence and genomic context and are present in meningococcal isolates which have persisted and spread globally. The absence of G4 and Sma/Cla sequences adjacent to the class II pilE genes is consistent with the lack of pilin subunit variation in these isolates, although horizontal transfer may generate class II pilin diversity. This study supports the suggestion that high frequency antigenic variation of pilin is not universal in pathogenic Neisseria.

Keywords:
Type four pilus; Neisseria meningitidis; Class I pilin; Class II pilin; Antigenic variation

Background

Neisseria meningitidis, the meningococcus, is an important human pathogen, being a leading cause of septicaemia and bacterial meningitis. Systemic disease has a high case fatality rate and can progress rapidly over the course of a few hours [1]. Different epidemiological patterns of meningococcal disease are observed across the world and Multi-Locus Sequence Typing (MLST) has been instrumental in characterising strains and identifying major pathogenic lineages (clonal complexes) responsible for disease [2]. Many N. meningitidis isolates from invasive disease belong to ‘hyperinvasive lineages’ [3], such as clonal complexes (cc)- 5, cc11, cc269, cc32 and cc41/44. Whole genome sequence (WGS) analyses have recently been carried out on large isolate collections, including those used to develop MLST [2,4]. These WGS data represent a valuable resource for investigating the frequency and variability of genes encoding meningococcal virulence traits.

Type four pili (Tfp) are important virulence factors for N. meningitidis, contributing to colonisation and disease [5]. Adhesion of meningococci to epithelial and endothelial cells is mediated by Tfp interacting with host cells, promoting bacterial aggregation and mediating microcolony formation [6]. Neisseria Tfp are also essential for the acquisition of exogenous DNA and emergence of strains with novel, heritable characteristics [7]. Along with LPS, porins and the polysaccharide capsule, Tfp have been considered as key meningococcal surface structures which undergo extensive variation. Studies on the Tfp of the related pathogen Neisseria gonorrhoeae have revealed that changes in the amino acid sequence of PilE (pilin), the major subunit of Tfp, arise through introduction of segments of non-expressed pilS cassettes into the pilE expression locus by a unidirectional DNA recombination event known as gene conversion [8,9]. This process is enhanced by the presence of the Sma/Cla repeat and requires the guanine quartet (G4) adjacent to the pilE expression locus. The Sma/Cla repeat is a 66 bp element present at the 3′ end of pilE that is proposed to bind proteins to facilitate recombination [10]. The G4 sequence is located upstream of pilE and is necessary for pilin conversion, putatively by acting as a recombination initiation structure [11,12]. More recently a cis-acting small RNA has been proposed to assist in the melting of the DNA duplex to allow formation of the G4 structure [13]. High frequency antigenic variation generates remarkable PilE diversity, with most of the sequence changes localised to the regions of the protein that are exposed on the surface of the pilus fibre [14], most notably within a region (D-region) located near the C-terminus of the protein. Gonococcal pilin variation has been detected both in vitro[15] and in human volunteer studies [16]. Meningococcal pilin variation has also been shown in a limited number of isolates in vitro[17] and in vivo[18,19] and has been proposed to influence Tfp-mediated adhesion [20-22], serum resistance [23] and to serve as a mechanism for immune evasion.

Two classes of Tfp (class I and class II) have been described in the Neisseria, based on the cross reaction of PilE with a monoclonal antibody, SM1 [24]. This antibody recognizes a conserved epitope in class I, but not class II pilin [25]. Furthermore, class II pilins lack most of the D-region typically seen in gonococccal and meningococcal class I pilins [26]. The class II pilE gene was initially described in the N. meningitidis serogroup C strain, FAM18 and subsequently found in non-pathogenic species, N. lactamica and N. cinerea[27]. Until recently, all pathogenic Neisseria were thought to have the ability to alter the sequence of the major pilin subunit through gene conversion; however, pilin variation was not detected in vitro in N. meningitidis strain FAM18, or strain NMB, which both express class II pilin [17,28]. Furthermore, in a previous study we found that cc8 and cc11 isolates of N. meningitidis collected from the UK over a period spanning 30 years harboured highly conserved class II pilE genes which lack variation characteristic of gene conversion [29]. Similar analysis of the sequence of pilE genes in meningococcal isolates from China demonstrated that strains in cc1 and cc5 also have a highly conserved class II pilE gene [30].

These findings suggest that high frequency pilE gene conversion is not universal in pathogenic Neisseria, an observation which has important implications for understanding the role of Tfp variation during colonisation, transmission and disease. Therefore, in this study we interrogated genome sequences of over 600 meningococcal isolates to further examine the distribution and sequence conservation of class II pilE genes, and to identify differences in the class I and class II pilE loci which might explain the lack of variability of the class II pilin gene. Our analyses showed that the class I and class II pilE genes are in distinct chromosomal contexts and that isolates with class II pilE contain a more limited repertoire of pilS cassettes. Furthermore, the G4 and Sma/Cla sequences, which are important for pilin variation, are absent from the class II pilE locus. Finally we present evidence of horizontal transfer of class II pilE genes between pathogenic and non-pathogenic Neisseria species, which has implications for the evolution of these bacteria.

Results

Distribution and diversity of class I and class II pilE in N. meningitidis

Draft whole genome sequences hosted on the PubMLST website [4] were interrogated to determine the distribution and diversity of class II pilE genes among N. meningitidis isolates. This included the Meningitis Research Foundation Meningococcus Genome Library (MRF-MGL), which contains the sequences of 514 disease-associated isolates from patients in England, Wales and Northern Ireland during a single epidemiological year (2010 to 2011), and which represents recently circulating UK disease causing meningococci. In addition WGS of the 107 strains of N. meningitidis used to develop and validate MLST, which comprises a diverse collection of meningococci isolated from 37 countries collected over a 59 year period (1937 to 1996) [2] were examined.

Pilin genes in this collection of isolates were identified by online BLAST analyses using full-length or partial class I or class II pilE sequences. Homologous sequences were manually extracted, translated and designated as either class I or class II pilE based upon relatedness to known pilin genes and the presence of the SM1 motif (EYYLN) in the translated sequence. It was not possible to identify full-length pilE sequences in all WGS, most likely due to incomplete assembly. From the MRF genomes a total of 201 full-length class I pilE coding sequences and 31 class II pilE coding sequences were identified and in the MLST collection of 107 genomes, 12 full-length class I pilE coding sequences and 44 class II pilE coding sequences were found (a breakdown of pilE analysis is described in Additional file 1). Therefore, in total 213 isolates with a full-length class I pilE gene and 75 isolates with a full-length class II pilE gene were identified (listed in Additional files 2 and 3). No isolates that contained both a class I and a class II pilE gene were identified.

Additional file 1. Breakdown of pilE analysis of genomes analysed in this study. Table and description of the identification of pilE genes in WGS analysed in this study.

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Additional file 2. N. meningitidis isolates with full length class I pilE. Table showing the ID, isolate name, year and country of isolation, sequence type and clonal complex information for all isolates with full length class I pilE identified in this work. The pilE allele ID is also shown.

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Additional file 3. N. meningitidis isolates with full length class II pilE. Table showing the ID, isolate name, year and country of isolation, sequence type and clonal complex information for all isolates with full length class II pilE identified in this work. The pilE allele ID is also shown.

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While there was no association of class II pilE with serogroup, country or year, the presence of a class II pilE gene was consistently associated with a limited group of clonal complexes i.e. cc1, cc5, cc8, cc11 and cc174; no WGS data from isolates belonging to these ccs contained a class I pilE gene. Only cc4 contained isolates with either class I pilE (ID 35 and ID 613) or class II pilE (ID 1) (see Additional files 2 and 3). Thus, the data from the 107 MLST and MRF isolate collections confirmed that the presence of class II pilin is a characteristic of a distinct subset of meningococcal lineages.

The extent of sequence diversity among the 213 class I and the 75 class II pilE sequences identified in this work was examined, including pilE sequences from eight available reference genome sequences, providing an additional six class I pilE genes and two class II pilE sequences (Additional file 4). Allele numbers were assigned to the full-length pilE (NEIS0210) coding sequences. Each of the 219 class I pilE genes was a unique allele (Table 1), even in strains of the same sequence type, isolated in the same country and year. In contrast, only 15 class II pilE alleles were identified from 77 meningococci isolated from 29 countries over 74 years, highlighting the sequence conservation of class II pilE genes (Table 1). There was also evidence for an association between certain class II pilE alleles and particular clonal complexes (Figure 1). For example, 90% (28/31) of cc11 isolates harboured an identical pilE (allele 4). Likewise, all 13 cc5 isolates harboured the same pilE coding sequence (allele 15). This allele was also found in strains belonging to cc1 and cc174, suggesting horizontal transfer of class II pilE. Further evidence of this was allele 14, which was present in strains belonging to cc1 and cc8.

Additional file 4. pilE allele in eight additional reference N. meningitidis isolates. Table showing the ID, isolate name, year and country of isolation, sequence type and clonal complex information for eight meningococcal isolates. These WGS are used as references as they are published as fully assembled, closed genomes. The pilE allele ID is also shown.

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Table 1. Analysis of class I and class II pilE sequence diversity

thumbnailFigure 1. Distribution of class II pilE nucleotide alleles among clonal complexes. A total of 15 different alleles were identified from 77 meningococcal isolates. Isolates from cc1 displayed the most diversity, with five unique pilE sequences found in 14 isolates. All 13 cc5 strains had identical pilE sequence. Alleles 14 and 15 were found in isolates from 2 or 3 different clonal complexes respectively. Clonal complex and pilE alleles are indicated.

Analysis of the predicted protein sequences showed that for class I pilins most variation occurred within the C-terminal D-region (Figure 2A), as expected based on previous studies [8,14,31]. The 219 different class I pilE alleles encode 219 different pilin subunits with 194 different D-regions (not shown). In contrast, alignment of the 15 unique class II pilins we identified demonstrates that variation occurs over the entire C-terminal domain and only six different D-regions are encoded (Figure 2B).

thumbnailFigure 2. Clustal W Alignment and Boxshade display of the deduced full-length amino acid sequences of (A) representative class I pilins from cc32, cc41/44 and cc269 and (B) the 15 class II pilin proteins encoded by 77 meningococcal isolates. For class I pilins, isolate ID and name are indicated. For class II pilins, sequence numbers correspond to the pilE allele number assigned by PubMLST. The phenylalanine (F) residue which is the start of mature pilin is shown by an arrow. The SM1 motif at the end of the putative N-terminal α-helical region is indicated. Black background highlights different residues. Grey background text indicates similar residues. The N-terminal region is highly conserved, as for all meningococcal type IV pilins. The D-region (highlighted) is defined by two conserved C-terminal cysteine residues. The hypervariable region of class I pilins is within the D-region. In class II pilins, sequence variation is found over the entire C-terminal domain.

Analysis of pilE/pilS loci in isolates with class I or class II pilE

In the class I pilin-producing meningococci with closed genome sequences (MC58 [32] and Z2491 [33]) the class I pilE gene and eight pilS cassettes are found in a single locus, while in the closed genomes of class II pilin-producing meningococci FAM18 [34] and WUE2594 [35], this locus contains a reduced number of pilS cassettes and class II pilE is located at a distinct locus (Figure 3). The class I pilE gene is located between fkbp (which encodes a putative peptidyl-prolyl cis-trans isomerase) and lpxC (UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase) (Figure 3A). The silent pilS cassettes precede pilE and, although there are eight cassettes in each instance, there is variation in their length and spacing. In contrast the class II pilE gene is situated between catalase (katA) and a hypothetical protein (hp), with a conserved hypothetical (chp) and putative oligopeptidase A gene (prlC) further downstream (Figure 3B). The catalase region in the genomes of MC58 and Z2491 does not contain a class II pilE gene (Figure 3A). Finally, in FAM18 and WUE2594, two or three pilS cassettes respectively are located in the region between fkbp and lpxC (Figure 3B).

thumbnailFigure 3. Schematic diagrams of the positions of pilE and pilS loci in genomes of reference strains of N. meningitidis expressing class I pilE (A) or class II pilE (B). Genes and pilS cassettes are drawn based on published annotations. The positions on the chromosome are based on NCBI reference sequences: NC_003112.2 (MC58), NC_008767.1 (FAM18), NC_003116.1 (Z2491), and NC_017512.1 (WUE2594). As shown, class II pilE genes have a distinct genomic context compared to the class I pilE genes. Putative guanine quartet (G4) and Sma/Cla sequences are shown as black vertical line or hatched boxes, respectively. lpxC: UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase), fkbp: peptidyl-prolyl cis-trans isomerase, katA: catalase, chp: conserved hypothetical protein; hp: hypothetical protein, prlC: putative oligopeptidase A.

To assess the chromosomal context of class I and class II pilE genes and their associated pilS repertoires in different groups of meningococcal isolates, we annotated the fkbp-lpxC region and the katA-prlC regions in a subset of WGS in the PubMLST database. Where possible groups of isolates with the same sequence type or in the same clonal complex were examined, to enable comparisons both within and between groups of related meningococci. The pilS cassettes were defined on the basis of their homology to pilE coding sequences. In some cases the lack of available contiguous sequence prevented identification of adjacent open reading frames; examples of class I and class II pilE/pilS regions from genomes where the assembly was sufficient are shown in Figure 4 and Additional files 5 and 6.

thumbnailFigure 4. Schematic diagrams of fkbp-lpxC and katA-prlC regions in a subset of meningococcal isolates with class I pilE (A-B) and class II pilE genes (C-D). (A) Open reading frames and pilS cassettes in the region flanking the class I pilE gene were annotated in isolates belonging to cc32. Class I pilE genes were mostly found adjacent to pilS cassettes in the fkbp-lpxC region. (B) The katA-prlC region of cc32 isolates does not contain a class II pilE gene. (C) Open reading frames and pilS cassettes were annotated in the fkbp-lpxC region of isolates belonging to cc5. These strains have three pilS cassettes which are highly conserved. (D) The class II pilE gene is located next to the catalase gene (katA) and is genetically linked to a gene encoding a hypothetical protein (hp). The ID and name of each isolate is indicated. Sequence type and provenance of each strain can be found in Additional files 2 and 3. Diagonal lines represent the end of the contig. X indicates the gene encodes an atypical pilin subunit which is unlikely to result in production of functional Tfp. Putative guanine quartet (G4) and Sma/Cla sequences are shown as black vertical line or hatched boxes, respectively. lpxC: UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase), fkbp: peptidyl-prolyl cis-trans isomerase, chp: conserved hypothetical protein; prlC: putative oligopeptidase A. Scale bars represent 1 kb.

Additional file 5. Schematic diagrams of class I pilE regions. Schematic representation of class I pilE/S regions and katA-prlC regions from isolates belonging to clonal complex (cc) 41/44 and cc262.

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Additional file 6. Schematic diagrams of class II pilE regions. Schematic representation of class II pilE regions and pilS regions from isolates belonging to cc1, cc8, cc174 and cc11.

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Class I pilE genes were found adjacent to pilS cassettes and between fkbp and lpxC in 29 of the 34 annotated genomes. In the remaining five genomes, it was not possible to determine whether pilE was adjacent to lpxC (Isolate IDs 20170 and 20382, Figure 4) or pilE genes encoding atypical pilin variants (with an extended and truncated C-terminus, respectively) were found, but these were not within in the fkbp-lpxC locus (cc32 isolates ID 20161 and ID 20250, Figure 4A). Likewise in cc269 isolate M10_240550 (ID 19986), the pilE/S locus was interrupted by a region homologous to the tspB region of strain FAM18 (see Additional file 5). None of these isolates harboured a pilE gene in the katA-prlC region (Figure 4B).

Analysis of pilS cassettes in the fkbp-lpxC region of the class I pilE-containing isolates revealed diversity in the spacing, number and sequence of the silent cassettes, both among clonal complexes and among isolates belonging to the same clonal complex (see Table 2 and schematic loci in Figure 4 and Additional file 5). This diversity was particularly evident in cc41/44, where we identified 52 different pilS sequences from a total of 84 cassettes from 12 genomes. It should be noted that the analysis of class I strains was specifically targeted to annotate pilS cassettes within the fkbp-lpxC region and the presence of additional pilS cassettes elsewhere in these genomes could not be excluded. Therefore this analysis represents the minimum diversity of pilS in isolates with class I pilE.

Table 2. pilS sequence diversity in isolates with class I and class II pilE1

The pilS and pilE sequences within each isolate were examined for evidence of past intra-strain gene conversion events. Of note, for all isolates with a class I pilE gene, it was possible to identify pilS cassettes with sequences corresponding to those found in the PilE D-region. In most cases the D-region was a hybrid, likely resulting from multiple recombination events, whilst in some instances a single pilS donor could be proposed based on full sequence identity (not shown).

Annotation of genomes with class II pilE revealed that this gene was present in a region defined as the katA-prlC region (annotated regions are shown in Figure 4C and D, and Additional file 6). All these regions had identical genetic organisation, except isolate 7891 (ID7, Finland, 1975), which lacks the conserved hypothetical protein (chp, NMC0207 in FAM18). Furthermore, all genomes with class II pilE had fewer pilS cassettes than class I isolates - usually two or three in the region between fkbp and lpxC (Figure 4C and Table 2). Further BLAST analyses of these class II genomes were performed to identify whether pilS cassettes were also in other loci. The only homologous sequences identified were located on contigs containing the region between fkbp and lpxC (not shown), suggesting that there was only a single pilS locus in these isolates. Although incomplete genome assembly preventing additional pilS identification in this study cannot be excluded, the fully assembled, closed genome sequences of reference strains FAM18 and WUE2594 do not contain pilS cassettes located elsewhere.

In comparison to strains with class I pilE, the pilS regions in these class II isolates were highly conserved within a clonal complex in terms of number and arrangement of pilS cassettes (see Figure 4 and Additional file 6). For example, there were only four different pilS sequences out of 64 cassettes in 32 cc11 isolates (Table 2). The pilS cassettes in class II isolates shared greater similarities with class I pilE than class II pilE as previously observed [36]. Furthermore, we were unable to identify any pilS cassettes encoding sequences corresponding to those found in the D-regions of any class II pilin proteins. This finding is consistent with the hypothesis that, in these class II isolates, pilS cassettes are not functioning as donors for intra-strain recombination events associated with pilin antigenic variation.

Analysis of DNA sequences associated with pilE gene conversion

The G4 and Sma/Cla elements have been shown to be required for efficient antigenic variation of pilin [12,37]. Each of these sequences was found in the region 5′ or 3′ to the class I pilE gene, respectively (Additional file 7). Four isolates lacked a full length Sma/Cla adjacent to pilE, three of which also presented atypical pilE sequence and/or arrangement; for example, cc41/44 isolate (ID19266) (Additional file 5), has an atypical pilE sequence and lacks a Sma/Cla sequence, while cc32 isolates ID 20161 and ID 20250 (Figure 4) have a Sma/Cla sequence adjacent to pilS but in these genomes pilE is no longer found in the fkbp-lpxC region.

Additional file 7. Sequences of putative G4 and Sma Cla sequences annotated in this study. Table of the G4 and Sma/Cla sequences identified in the pilE/pilS regions of WGS annotated in this study.

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We also analysed the regions flanking the class II pilE alleles for presence of the Sma/Cla and G4 sequences. We did not detect sequences corresponding to a functional G4 motif (5′-GGGTGGGTTGGGTGGG-3′) [12] in the region 5′ to any class II pilE gene. Furthermore, the Sma/Cla repeat was absent from the region 3′ to the class II pilE genes, although sequences identical or highly homologous to the published meningococcal Sma/Cla repeat [37] were present in the pilS loci of these isolates (see Figure 4 and Additional files 6 and 7). Therefore, the absence of motifs that are important for pilE/pilS recombination adjacent to the class II pilE gene is likely to contribute to the lack of antigenic variation of class II pilE.

Defining meningococcal class II pilins

Class II pilins were initially identified by their lack of cross reaction with monoclonal antibody SM1 which was developed to differentiate between structural classes of Tfp on gonococci and meningococci [24]. SM1 recognises an epitope (EYYLN) present at the end of the conserved N-terminus of class I pilin [25]. Our analysis confirms that all the pilins encoded by the class I pilE contain this motif, while in the class II pilins this motif is either EYYSN (allele 15, present in 20 isolates) or EYYSD (present in all remaining isolates) (Figure 2). Western blot analysis of whole cell extracts from strains expressing class II pilin subunits with different SM1 sequences was undertaken (Figure 5). As expected, SM1 detected class I pilin in the control strain 8013 (ID 1038, allele 6, EYYLN motif) and did not recognise the class II pilin from FAM18 (ID 698, allele 3, EYYSD motif). However, class II pilin from cc5 strain 92001 (ID 597, allele 15, EYYSN motif), does cross react with SM1. This suggests that SM1 reactivity may not be sufficient to distinguish class I and class II pilin in all meningococci. We therefore compared all the pilin sequences from our analysis to examine the presence and conservation of additional differentiating features (Table 3). The sequence of the leader peptides were distinct (MNTLQKG and MKAIQKG for class I and II pilins respectively) as was the length of the C-terminus (after the second conserved cysteine), which comprises one to three amino acids in class II pilins, in contrast to seven in full-length, functional class I pilins. Most notably, all pilins encoded by the class II pilE genes were smaller, mostly due to a significantly shorter D-region, which is 13 or 16 amino acids in length compared with 28 to 40 amino acids in class I pilins (see Figure 2 and Table 3). Based on this analysis, we propose the adoption of a sequence-based distinction between class I and class II pilins, rather than a definition based on antigenic properties (reactivity with SM1).

thumbnailFigure 5. Western blot analysis of SM1 cross reactivity with class I and class II meningococcal pilins. Whole cell extracts from strains with class I or class II pilE genes encoding pilins with different SM1 epitopes, and isogenic mutants lacking pilE were analysed using monoclonal antibody SM1 and anti-peptide antibody EP112606. All strains express pilin, as detected by EP112606. As expected, SM1 reacts with class I pilin in 8013 but does not recognise the class II pilin from FAM18. However, pilin from isolate 92001 which has a class II pilE gene (allele 15) also cross reacts with SM1.

Table 3. Defining features of meningococcal class I and class II type IV pilins

Analysis of pilE in non-pathogenic Neisseria species

It has previously been shown that class II pilE is also present in non-pathogenic Neisseria[27,36,38]. We therefore analysed pilE sequences in genomes of 46 isolates of non-pathogenic Neisseria available in pubMLST. This collection comprised 14 different species isolated between 1962 and 2003 from various hosts and sites. In total 44/46 WGS from non-pathogenic Neisseria were found to have full-length sequences homologous to pilE (These isolates are listed in Additional file 8). Split decomposition network analysis of these 44 pilE sequences with both class I and class II pilE genes from 14 N. meningitidis isolates from different clonal complexes, revealed that all meningococcal class I pilE clustered together (Figure 6). In contrast, the meningococcal class II pilE clustered on a separate branch together with pilE from N. lactamica, N. polysaccharea and one N. cinerea isolate. Other non-pathogenic Neisseria species displayed more divergent pilE sequences. We analysed the sequences flanking the putative pilE genes in the genomes of all non-pathogenic Neisseria strains where there was sufficient sequence available on the pilE-containing contig. Nine species contained a second putative pilin gene adjacent to the initial pilE homologue (see Additional file 8), in agreement with previous reports [36,38] and in these species the regions flanking pilE did not resemble those found adjacent to either the class I or class II meningococcal pilE. In contrast, the genetic organisation of open reading frames around the pilE gene in N. lactamica and N. polysaccharea was similar to the genomic region surrounding the meningococcal class II pilE gene (annotated pilE regions of N. lactamica and N. polysaccharea are shown in Additional file 9), although only one non-pathogenic Neisseria isolate (N. polysaccharea, isolate CCUG 27182) was found to harbour the hp gene which is found in the meningococcal class II pilE locus.

Additional file 8. Non-pathogenic Neisseria isolates examined in this study. Table describing the origin and details of the non-pathogenic Neisseria WGS analysed in this work. The number of putative pilE genes identified is also indicated.

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thumbnailFigure 6. SplitsTree decomposition network of the pilE gene in different Neisseria species. Full-length pilE coding sequences were obtained from 44 representative non-pathogenic Neisseria genomes available in PubMLST. The pilE from pathogenic Neisseria included in this analysis were from reference strains (i.e. those with fully assembled, closed genomes). For comprehensive analysis we also included meningococcal class II pilE from isolates belonging to cc1, cc4 and cc174 obtained in this work. The species are indicated by colour code. Meningococci with class I pilE (black circles) form a separate branch, while the meningococcal class II pilE genes (black squares) are more closely related to the pilE from non-pathogenic Neisseria species, N. polysaccharea (white circles), N. lactamica (red circles) and one isolate of N. cinerea (grey circle).

Additional file 9. Schematic diagrams of pilE regions of N. lactamica and N. polysaccharea. Schematic representation of pilE regions in N. lactamica and N. polysaccharea WGS analysed in this study.

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Discussion

Two different classes of pilin (class I and class II) have been described in pathogenic Neisseria[24] and the genes encoding these pilins are distinguishable [29,39]. Here we have taken advantage of available whole genome sequences to provide a more comprehensive view of the distribution and genetic context of meningococcal class I and class II pilE. These analyses reveal a possible basis for the evident lack of gene conversion of class II pilE and we provide insights into the likely origin of the meningococcal class II pilE gene.

Previous analysis of pilE sequences in N. meningitidis isolate collections from the UK [29] and China [30] suggested an association of the class II pilE gene with certain lineages, especially those responsible for epidemic meningococcal disease (i.e. cc1, cc5 and cc11). In the present analysis, two large and well characterised isolate collections enabled the assessment of class II pilE distribution in isolates from a single country in one year, and in isolates collected over decades from diverse global locations. The data demonstrate that the association of class II pilE with these hyperinvasive lineages is maintained, regardless of the country or the year they were isolated, and highlights that class II pilE is present in lineages which are responsible for a large proportion of disease. For example, the 107 isolate MLST collection analysed in this study contains cc1 and cc5 isolates from countries of the African meningitis belt (ID 128 and ID 34, Additional file 3). The majority of epidemic meningococcal disease has occurred in this region and has been mostly caused by serogroup A meningococci belonging to cc1 or cc5 [40,41]; in particular three clones (ST-5, ST-7 and ST-2859, which are all part of cc5) have been largely responsible for meningococcal disease in Africa over the last 20 years [42-44].

In addition, we found class II pilE genes in strains from cc174 and cc4. An increase in carriage of cc174 strains was reported in the UK from 1999-2001 [45] and isolates from cc174 accounted for approximately 20% of serogroup Y meningococcal disease during 2007-2009 in the UK and were associated with disease in older age groups [46]; however, only 9/514 disease isolates from the UK in 2010-2011 belong to this complex. Therefore, there is currently insufficient information to determine whether strains belonging to this clonal complex are hyperinvasive and/or associated with epidemic disease. It is possible that the groups of strains with class II pilE may expand with increasing availability of strain collections and whole genome sequences and consequently the association of class II pilE with strains that have particular epidemiological characteristics should become clearer.

A class II pilin gene was identified in one isolate from cc4, which was of particular interest as we also identified isolates in this clonal complex that possess a class I pilE gene (see Additional file 10). This was inconsistent with our previous analysis which indicated that isolates within clonal complexes were uniform with respect to pilin class (i.e. they all have either class I or class II pilE). cc4 meningococci are reported to be genetically homogeneous and were responsible for epidemics in the meningitis belt from the 1960s to the early 1990s [47]. The cc4 isolate with class II pilE was the oldest strain in the collection, isolated in 1937, while the two strains with class I pilE were from 1963 and 1983, suggesting that there may have been a shift from class II pilE to class I pilE in cc4. It is interesting to note that the cc4 complex has been replaced as the major cause of disease in the meningitis belt by cc1 or more recently cc5 meningococci, both of which have class II pilE[47].

Additional file 10. Schematic diagram of pilE regions in cc4 isolates. Schematic representation showing the genetic context of the class I or class II pilE genes identified in cc4 meningococcal isolates.

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Our analysis confirms that class II pilins in meningococci are antigenically stable. For example, we found that a serogroup W isolate (ID 20449, M11_240427, cc11) responsible for disease in the UK in 2011, has an identical pilE sequence to a serogroup B isolate (ID 349, 38VI, cc11), isolated from the USA in 1964. This contrasts with the finding that every class I pilin had a different sequence, even from strains with the same sequence type, isolated from the same country, during the same year. The extensive variation of class I pilE is consistent with the ability of single strains to generate pilin diversity through the recombination between pilS cassettes and the pilE expression locus [48]. Indeed, for the majority of isolates with class I pilE the putative donor pilS cassettes could be identified. Although pilS cassettes were found in all isolates which harboured a class II pilE gene, it was not possible to identify any class II pilE sequences that contained D-region information corresponding to pilS cassettes. Therefore, the WGS analysis provides further evidence for a lack of pilE/S recombination in class II pilin producing meningococci. A possible explanation for the low variability of the class II pilE genes is the absence of the G4 sequence that is required for pilin conversion. The current hypothesis is that the formation of a guanine quartet on the lagging strand stalls DNA replication, leading to a nick on the leading strand and initiating antigenic variation [49]. Therefore, in the absence of a functional G4 adjacent to pilE, these strains would lack the ability to initiate pilin conversion, although recent work suggests that additional factors may contribute to the lack of pilin variation [28]. In this respect, isolates expressing class II pilin provide a useful tool for systematically dissecting the molecular and genetic elements required for pilin variation.

These results also raise questions concerning the origin of the meningococcal class II pilE locus. The analysis of pilE genes in pathogenic and non-pathogenic Neisseria indicates that the N. meningitidis class II pilE is more closely related to pilE from non-pathogenic species than to the class I pilE from meningococci, in agreement with previous reports [27,36]. These observations are consistent with horizontal gene transfer among Neisseria species which has been observed by others [38,50] and is likely, given that diverse species share a biological niche within the human host. Furthermore, we found one isolate of N. polysaccharea with the hp gene adjacent to pilE. This gene, which encodes a putative transposase from Haemophilus influenzae, is consistently found next to the class II pilE gene in meningococci. Therefore this provides additional evidence in support of genetic exchange around the pilE locus among these species.

In addition to differences in pilE, meningococci producing class II pilin had fewer pilS cassettes (1-4 pilS) in the region between lpxC and fkbp than isolates with class I pilin (4-11 pilS). One possible explanation for this finding is that the lpxC-fkbp region of existing class II strains represents a remnant of an ancestral class I pilE/pilS region, and that these class II isolates have at some point acquired a class II pilE gene and lost the class I expression locus and some pilS cassettes. Interestingly, no genomes with both a class I and a class II pilE gene were found in this study. It is also noteworthy that recent genomic analysis indicates that pathogenic Neisseria evolved from a common ancestor shared with N. polysaccharea[50], which has a class II pilE gene. This could suggest a second possible scenario in which the class II pilE locus is ancestral in N. meningitidis and the class I pilS/pilE locus is a more recent trait which has arisen in specific lineages.

Tfp are surface structures which are exposed to the host environment in both colonisation and disease, and are thus presumed targets of the immune system. Consequently pilin variation has largely been assumed to provide meningococci with an effective mechanism of immune evasion. Indeed, anti-pilus antibodies can be found in patients infected with N. meningitidis[51] and gonococcal Tfp containing class I pilin are immunogenic in human volunteer studies [52,53] although there is no clear role for anti-PilE antibodies in protection against meningococcal infection [54]. Our finding that some clonal complexes express conserved class II pilin subunits is not consistent with host immunity-driven pilin variation in these strains, and is reminiscent of the lack of diversification of other surface proteins such as PorB, PorA, and FetA in some hyperinvasive strains [55,56]. However, we found some evidence of class II pilE gene exchange between unrelated isolates (i.e. different clonal complexes), indicating that recombination following uptake of exogenous DNA is a potential mechanism of generating class II pilin variation. A better understanding of the immune responses to class I and class II pilins should provide insight as to why distinct hyperinvasive lineages have evolved different potential for sequence variation of the Tfp major subunit.

Conclusions

In conclusion, we show that class II pilins can be defined by amino acid sequence and genomic context and are present in N. meningitidis strains which have persisted and spread globally over several decades and are responsible for meningococcal disease worldwide. Furthermore the class II pilE gene is present in non-pathogenic Neisseria, including N. polysaccharea and N. lactamica. Despite the presence of pilS cassettes as potential donors of genetic variation in meningococci producing class II pilins, we found no evidence that class II pilE recombines with pilS to generate pilin variation. Given the importance of Tfp in pathogen-host interaction, we propose that possession of class I or class II pilE is likely to impact bacterial behaviour, either through different capacity of strains to alter the sequence of the major pilus component or directly, through the inherent properties of the specific pilin subunits. For example, particular sequences of class I pilins affect key Tfp functions by influencing cell tropism or adhesion [20,57,58]. Additionally, specific amino acids in the D-region can affect pilus bundling [59] and this has recently been linked to serum resistance [23]. It is possible that class II pilins are highly conserved in certain lineages because they confer specific properties important for interactions within the host. Future work will be directed at understanding how these different pilin genes have evolved and their impact upon meningococcal biology.

Methods

Genome database and collections

The genome sequences of Neisseria strains used in this work are publically available online in the PubMLST Neisseria BIGSdb database (http://pubmlst.org/neisseria/ webcite). This database was developed by Keith Jolley and is sited at the University of Oxford [4]. The MRF-MGL (http://www.meningitis.org/research/genome webcite) is an online open access library developed collaboratively by the Health Protection Agency, the Wellcome Trust Sanger Institute and the University of Oxford and is also hosted on the PubMLST website. The sequences analysed in this work were, for the most part, draft whole genome sequences and the majority were not fully assembled or closed genomes. The number and length of contigs for genomes can be found by using the “sequence bin” analysis tool on the PubMLST website. The 107 isolate MLST collection was accessed via the “Search Neisseria PubMLST database” web page using the “Publication” filter and selecting “Maiden et al. [2], Proc Nat Acad Sci USA 95:3140-5” from the drop down menu. The genomes of 514 meningococcal disease isolates from the epidemiological year 2010/11 in England, Wales and Northern Ireland were available in the MRF genome library (at time of writing). The genomes of 46 other Neisseria species were selected by excluding species names containing meningitidis and gonorrhoeae, and filtering for isolates with whole genome sequences in the drop down project menu. Each isolate has a unique ID number. Fully assembled (closed) genome sequences for reference strains MC58 (ID 240) [32], FAM18 (ID 698) [34], WUE2594 (ID 19260) [35], G2136 (ID638) [60], α14 (ID 30) [61], α710 (ID 19261) [62], M01-240355 (ID 19265) [60] , NZ-05/33 (ID 19263) [60], H44/76 (ID 237) [63], 8013 (ID 1038) [64] and N. lactamica 020-06 [65] are described in previous publications and are available on PubMLST.

Identification of class I and class II pilE genes in meningococcal genomes

Homologs of pilE were identified in the selected genomes using the BLASTn online analysis tool in PubMLST. For initial searches, the default parameters (word size 11 and 1 hit per isolate) were used. The query sequence consisted of either the full-length coding sequence of previously identified class I or class II pilE genes (e.g. MC58 [32] [Genbank: NC_003112] and FAM18 [26], [Genbank: NC_008767]) respectively or unpublished class II pilE sequences - R. Exley). The percentage identity between the nucleotide sequence of pilE from MC58 (class I) and FAM18 (class II) is 69% (Clustal W), and based on previous work [29] class II pilE genes are significantly shorter than class I pilE genes (approx. 450 bp compared with ≥ 500 bp) therefore in most instances using a class I or a class II pilE coding sequence was sufficiently stringent to distinguish either a class I or a class II pilE homolog. However, to verify our findings, more comprehensive searches were also performed where the query sequence comprised nucleotides 1-180 of the pilE coding sequence from serogroup B strain MC58, which corresponds to the highly conserved N-terminal alpha helical region of all meningococcal Type IVa pilins [66]. Hits with >80% sequence identity over an alignment length of ≥50% of the query sequence were extracted with flanking sequence and manually inspected to identify pilE coding sequences and classify them as class I or class II based on the known characteristics of class I or class II pilins e.g: size, presence of the SM1 epitope EYYLN for class I pilins [25,26]. Using these search parameters we identified some genomes where pilE was not detected (Additional file 1). Given that the majority of the genome sequences are represented on contigs but are not closed, it was not possible to determine whether this was due to absence of pilE or insufficient contig assembly, therefore, these were excluded from further analysis. Additionally we excluded pilE sequences that were truncated by contig length as it was impossible to ascertain whether these were class I or class II pilE in the absence of full-length coding sequence. Finally, we also identified pilE genes that were either elongated, truncated by frameshift or had deletions compared to full-length pilE coding sequences. These sequences most likely resulted from gene conversion and would likely lead to production of a non-functional pilin subunit [67] and thus were also not included in further sequence analysis in this study. pilE was designated as NEIS0210 in PubMLST and each new nucleotide allele identified was assigned a unique number in the PubMLST definitions database. To identify whether any genomes contained more than one pilE gene, BLAST searches were performed using class I and class II pilE as query sequences, as well as selecting for up to 10 hits per isolate.

Identification of pilE genes in non-pathogenic Neisseria

Homologs of pilE were identified in selected non-pathogenic Neisseria genomes using the BLASTn online analysis tool in PubMLST. For initial searches, the default parameters (word size 11, 1 hit per isolate and 5000 bp flanking length) were used. The query sequence consisted of the full-length pilE coding sequence of N. lactamica 020-06. Sequences were extracted and manually inspected to identify pilE. In species where two putative pilin genes were identified in tandem on the same contig, the gene with the higher homology to the N. lactamica 020-06 pilE was chosen for split decomposition network analysis.

Identification of pilS cassettes in isolates containing class I pilE

We analysed pilin loci in a subset of cc41/44, cc32, and cc269 isolates in order to determine the number and diversity of pilS cassettes residing in each pilE/S locus. We initially extracted the pilE/S locus from each strain by performing BLASTn searches in PubMLST using pilE as the query sequence; all search conditions were at default setting with the exception of a designated flanking length of 10,000 bp. The pilE-containing contigs were copied to serial cloner files and fkbp and lpxC genes were identified based on homology to previously annotated or published sequences. Sequences where contig length was insufficient to identify flanking genes were excluded from further analysis. We next identified individual pilS cassettes within the fkbp-lpxC regions of each isolate by utilizing the Align Two Sequences tool available at NCBI to interrogate the extracted fkbp-lpxC region using the pilS1 gene from N. meningitidis strain M01-240149 as a query sequence in tBLASTn searches performed under default conditions. Each hit with >60% sequence identity was inspected manually for features characteristic of known neisserial pilS cassettes, which have been defined as partial genes with homology to pilin-encoding sequences [31,68]. We delineated the 5′ and 3′ boundaries of each pilS cassette based on this criterion, although in some cases open reading frames extended beyond the region of pilin homology.

Identification of pilS cassettes in class II pilE-containing isolates

The genome sequence of each isolate with class II pilE was first examined for pilS-containing regions using the BLASTn online analysis tool on PubMLST using pilS1 and pilS2 genes from N. meningitidis strain FAM18 as query sequences under default conditions. Sequence regions containing hits were extracted with up to 5000 bp of flanking sequence as allowable by contig length. We examined each extracted pilS-containing region for fkbp homology, lpxC homology, and number and diversity of pilS cassettes as indicated above. Comparisons of pilE and pilS sequences from each strain were also carried out as indicated above.

Identification of Sma/Cla and Guanine quartet sequences

The sequence of the 66 bp Sma/Cla repeats from N. gonorrhoeae and N. meningitidis strain MC58 reported in [10,37] were used as a query sequences to scan pilE loci for identical sequences. Where identical sequences were not identified, we manually inspected loci for highly similar sequences. Of note, the SmaI (CCCGGG) and ClaI (ATCGAT) recognition sites were not present in the query sequence and are not consistently present flanking this repeat in all genomes. However, for consistency we have kept the name Sma/Cla repeat. The sequences reported in Cahoon and Seifert [12] were used to identify putative G4 based on sequence homology.

Sequence analysis, annotation and illustration

The online sequence analysis tool NRDB (http://pubmlst.org/analysis/ webcite) was used to identify unique alleles and EMBOSS transeq (http://www.ebi.ac.uk/Tools/st/emboss_transeq/ webcite) was used to translate multiple coding sequences for protein sequence analysis. Nucleotide and amino acid alignments, including comparisons of pilE and pilS loci from each isolate, were carried out using the online analysis tool MUSCLE available at EBI [69] or Clustal W (http://www.ebi.ac.uk/Tools/msa/clustalw2/ webcite) [70]. Alignment was displayed using the BoxShade 3.31 program on the Mobyle platform [71]. Genome regions were annotated by performing BLAST analysis with pilE (class I or class II), or alternatively fkpb or lpxC as the query sequence and downloading flanking regions (usually up to 10,000 bp) from the pubMLST website. Coding sequences on the contigs were identified based on homology to previously published and/or annotated sequences [32-34,64,72,73]. Gene diagrams of regions between fkbp and lpxC and katA and prlC by were manually constructed and drawn to scale using Microsoft PowerPoint.

Phylogenetic network analysis

The SplitsTree decomposition network of pilE sequences was obtained by performing sequence alignment in MEGA 5.0 [74] and network analysis using Splitstree4 [75]. Full-length nucleotide sequences of pilE homologues from non-pathogenic Neisseria species were compared with pilE genes from 14 N. meningitidis isolates which are reference isolates with previously published and annotated whole genomes (listed in Additional files 2, 3 and 4), and/or represent lineages with class II pilE identified in this work (i.e. cc1, cc4 and cc174).

Western blot analysis of SM1 cross reactivity of class II pilins

N. meningitidis strains FAM18 (ID 698), 8013 (ID 1038) and 92001 (ID 597) and isogenic mutants in which the pilE coding sequence is replaced by a kanamycin resistance cassette were grown overnight on Brain Heart Infusion (BHI) agar (1.5% wt/vol, Oxoid) at 37°C in the presence of 5% CO2. Bacteria (1 × 109 CFU) were resuspended in 50 μl of sterile water and an equal volume of 2× SDS-PAGE lysis buffer was added before boiling for 10 minutes. 10 μl of each whole cell extract was separated on 12% polyacrylamide gels and transferred to PVDF membrane for Western blotting. SM1 antibody (a kind gift from M. Virji) was used at a final concentration of 1:10,000 and antibody binding was detected by incubation of membranes for one hour with a goat anti-mouse HRP conjugate (DAKO) at 1:10,000. An anti-peptide antibody generated by immunisation of rabbits with peptide CGQKSAVTEYYLNHGE (Eurogentec) was used to detect both class I and class II pilin expression at a final concentration of 1:5000, followed by an anti-rabbit HRP conjugate (1:10,000; Santa Cruz). Cross reaction was detected using ECL detection reagent (GE Healthcare).

Availability of supporting data

The draft or complete genomes available in this article are publically available online as described. Additional files with supporting results are included with this article.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

RE and CT conceived the idea for this article. MW and RE analysed the pilE sequences, CH and EA analysed pilS loci, MW and JB analysed pilE sequences from non-pathogenic Neisseria, KJ and MM devised the PubMLST database and contributed to all aspects of data extraction and analysis. RE, EA, CT, MW wrote the manuscript. All authors read and approved the final submission.

Acknowledgements

RE, MW and CT are funded by the Wellcome Trust (WT093470AIA). MCJM is a Wellcome Trust Senior Research Fellow in Basic Biomedical Sciences. The Meningitis Research Foundation Meningococcus Genome Library was funded by the Meningitis Research Foundation. The Neisseria Multi Locus Sequence Typing website was funded by the Wellcome Trust and European Union. We thank Lydia Stinar and Stephen Rostad for assistance with the pilS analysis.

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