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Open Access Research article

Western corn rootworm (Diabrotica virgifera virgifera) transcriptome assembly and genomic analysis of population structure

Lex E Flagel1, Raman Bansal2, Randall A Kerstetter1, Mao Chen1, Matthew Carroll1, Ronald Flannagan1, Thomas Clark1, Barry S Goldman1* and Andy P Michel2*

Author Affiliations

1 Monsanto Company, 700 Chesterfield Parkway W, Chesterfield, MO 63017, USA

2 Department of Entomology, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691, USA

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BMC Genomics 2014, 15:195  doi:10.1186/1471-2164-15-195

Published: 14 March 2014

Abstract

Background

Western corn rootworm (WCR) is one of the most significant insect pests of maize in North America. WCR has dramatically increased its range in the last century, invading key maize production areas in the US and abroad. In addition, this species has a history of evolving traits that allow it to escape various control options. Improved genetic and genomic resources are crucial tools for understanding population history and the genetic basis of trait evolution. Here we produce and analyze a transcriptome assembly for WCR. We also perform whole genome population resequencing, and combine these resources to better understand the evolutionary history of WCR.

Results

The WCR transcriptome assembly presented here contains approximately 16,000 unigenes, many of which have high similarity to other insect species. Among these unigenes we found several gene families that have been implicated in insecticide resistance in other species. We also identified over 500,000 unigene based SNPs among 26 WCR populations. We used these SNPs to scan for outliers among the candidate genes, and to understand how population processes have shaped genetic variation in this species.

Conclusions

This study highlights the utility of transcriptomic and genomic resources as foundational tools for dealing with highly adaptive pest species. Using these tools we identified candidate gene families for insecticide resistance and reveal aspects of WCR population history in light of the species’ recent range expansion.

Keywords:
Diabrotica virgifera virgifera; Insect resistance; Transgenic crop; Transcriptome; Western corn rootworm