Open Access Research article

Contribution of natural antisense transcription to an endogenous siRNA signature in human cells

Andreas Werner1*, Simon Cockell2, Jane Falconer3, Mark Carlile4, Sammer Alnumeir1 and John Robinson5

Author Affiliations

1 RNA Biology Group, Institute of Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle NE2 4HH, UK

2 Bioinformatics Support Unit, The Medical School, Newcastle University, Framlington Place, Newcastle NE2 4HH, UK

3 School of Life Sciences, Northumbria University, Ellison Place, Newcastle upon Tyne NE18ST, UK

4 Faculty of Applied Sciences, University of Sunderland, Wharncliffe Street, Sunderland SR1 3SD, UK

5 Institute of Cellular Medicine, Newcastle University, Framlington Place, Newcastle NE2 4HH, UK

For all author emails, please log on.

BMC Genomics 2014, 15:19  doi:10.1186/1471-2164-15-19

Published: 13 January 2014

Additional files

Additional file 1: Table S1:

Compilation of genes producing short RNAs.

Format: PDF Size: 316KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2: Figure S1:

Genes with short reads compared to the total number of genes.

Format: PDF Size: 98KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Figure S2:

Expression of endo-siRNA related genes.

Format: PDF Size: 146KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4: Table S2:

Expression data of miRNAs and structural RNAs.

Format: PDF Size: 400KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5: Figure S3:

Length distribution of the short RNAs in the three samples control, clone 5 and clone 12.

Format: PDF Size: 100KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 6: Table S3:

Summary of qPCR results.

Format: PDF Size: 400KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 7: Table S4:

Details and sequences of primers and probes for qPCR.

Format: PDF Size: 32KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data