Table 2

Overall mismatch rates in overlapping paired-end reads for raw read pairs, read pairs that have quality scores Q10, Q≥20, Q≥30 and Q≥35
Sample Raw Q10 (1×10-1) Q20 (1×10-2) Q30 (1×10-3) Q35 (3×10-4)
BCV control 5.55×10-3 7.82×10-4 9.56×10-5 1.94×10-6 4.26×10-8
Rabies control 7.33×10-3 9.64×10-4 1.12×10-4 2.59×10-6 1.31×10-7
BCV 4.91×10-3 7.87×10-4 1.06×10-4 2.55×10-6 1.51×10-7
Fox1 8.92×10-3 1.01×10-3 1.73×10-4 8.82×10-6 6.92×10-7
Fox2 6.69×10-3 8.33×10-4 1.22×10-4 4.16×10-6 2.28×10-7
Average of 5 samples 6.68×10-3 8.75×10-4 1.22×10-4 4.01×10-6 2.49×10-7
Rabies control –repeat run 5.99×10-3 2.90×10-3 1.04×10-3 2.67×10-4 8.00×10-5

The rabies control plasmid was sequenced a second time using a separate protocol at a different service provider and listed here for comparison. Mismatch rates are ‘per base pair’ so that they can be compared directly across samples and platforms. Phred error rates for the Q scores are shown in the heading.

Chen-Harris et al.

Chen-Harris et al. BMC Genomics 2013 14:96   doi:10.1186/1471-2164-14-96

Open Data