Table 2

Microarray results of transcripts demonstrating greater than three-fold difference in expression between Jund siRNA and scrambled control siRNA transfected WKY BMDMs
Gene symbol Gene name Chr. Fold change FDR (%) Gene Function
Basal condition
Klrb1a killer cell lectin-like receptor subfamily B member 1B 4 9.0 <0.01 IL-1β synthesis [19]
Mt2A metallothionein 2A 19 4.6 0.63 Oxidative stress [15]
Il1b interleukin 1 beta 3 4.1 2.85 IL-1β synthesis
Cxcl9 chemokine (C-X-C motif) ligand 9 14 3.5 <0.01 Inflammation
Lcn2 lipocalin 2 3 3.3 <0.01 Oxidative stress [16]
Vcan versican 2 3.3 <0.01 Oxidative stress [17]
Nlrp3 NLR family, pyrin domain containing 3 10 3.2 3.01 IL-1β synthesis [21]
D3ZIY9_RAT uncharacterised protein 10 3.1 1.09 -
Dot1l DOT1-like, histone H3 methyltransferase (S. cerevisiae) 7 -3.7 2.30 Inflammation
Hspb1 heat shock protein 1 12 -3.8 <0.01 Oxidative stress [18]
Prkca protein kinase C, alpha 10 -4.1 1.83 Oxidative stress [19]
LPS stimulated condition
Klrb1a killer cell lectin-like receptor subfamily B member 1B 4 4.6 <0.01 IL-1β synthesis [20]
Hspb1 heat shock protein 1 12 -4.0 <0.01 Oxidative stress [18]

Differentially expressed transcripts with greater than three-fold difference in expression (four technical replicates per strain) were identified at a <5% FDR threshold following 40,000 permutations. Fold changes are of control siRNA versus Jund siRNA expression. A positive fold change indicates higher expression in BMDMs transfected with scrambled control siRNA i.e. with a higher level of Jund expression compared to Jund siRNA. Abbreviations: Chr.; chromosome, FDR: false discovery rate.

Hull et al.

Hull et al. BMC Genomics 2013 14:92   doi:10.1186/1471-2164-14-92

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