siRNA mediated knockdown of Jund. Assessment of the efficiency of siRNA knockdown of Jund in the unstimulated state (A, C) and following eight hours LPS stimulation (B) using qRT-PCR and Western blotting. siRNA experiments were performed in 4 different WKY rats in triplicate. ***P<0.001 using two tailed unpaired t-test to compare BMDMs transfected for 48 hours with either scrambled control siRNA or Jund siRNA. The Western blot (C) for JunD is representative of four different Jund silencing experiments in WKY BMDMs and is demonstrated alongside β-actin loading control.
Hull et al. BMC Genomics 2013 14:92 doi:10.1186/1471-2164-14-92