Quality control of fragmented DNA. (A) Bioanalyzer results from three DNA samples fragmented either in glass tubes with a Covaris DNA shearing device (Duty cycle 10%, Intensity 4.5, Cycles per burst 200, Time 120 s), or in PCR strips with a Bandelin sonicator (2 times 4 min). (B) 1.5% agarose gel loaded with 22 samples fragmented by Bandelin sonication. The size distribution is very narrow (major peak between 100–300 bp) and has an acceptable reproducibility.
Wilkening et al. BMC Genomics 2013 14:90 doi:10.1186/1471-2164-14-90