Open Access Highly Accessed Methodology article

Genotyping 1000 yeast strains by next-generation sequencing

Stefan Wilkening1, Manu M Tekkedil1, Gen Lin1, Emilie S Fritsch1, Wu Wei1, Julien Gagneur2, David W Lazinski3, Andrew Camilli3 and Lars M Steinmetz1*

Author Affiliations

1 Genome Biology Unit, European Molecular Biology Laboratory, Meyerhofstr. 1, 69117, Heidelberg, Germany

2 Gene Center Munich, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Str. 25, 81377, Munich, Germany

3 Department of Molecular Biology & Microbiology and Howard Hughes Medical Institute, Tufts University, 136 Harrison Avenue, Boston, MA, 02111-1817, USA

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BMC Genomics 2013, 14:90  doi:10.1186/1471-2164-14-90

Published: 9 February 2013

Additional files

Additional file 1: Tables S1:

Summary of publications on DNA-Seq improvements, Tables S2. Quality measures for different library preparations, Tables S3. Oligonucleotide sequences, Tables S4. Recombination sites in SK1xS96 segregants, Tables S5. List of 20 regions with biggest differences between recombination frequency between S96xYJM789 and SK1xS96 set.

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Additional file 2: Figures S1:

IGV example for regions with extreme GC content, Figures S2. Barcode amplification bias, Figures S3. Recombination frequency around Spo11 oligo hotspots, Figures S4. Detection and classification of missegregation, Figures S5. Correlation between meiotic disomy and structural variations, Figures S6. Photo of self-made magnetic stand.

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Additional file 3:

bmf files (zipped files including + protocol).

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Additional file 4: Video S1:

Showing fragmentation by Bandelin sonication.

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