Open Access Research article

Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations

Cristina Crosatti2, Lydia Quansah1, Caterina Maré2, Lorenzo Giusti2, Enrica Roncaglia4, Sergio G Atienza3, Luigi Cattivelli2* and Aaron Fait1*

Author Affiliations

1 Jacob Blaustein Institutes for Desert Research, French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990 Sde Boqer, Israel

2 Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d’ Arda, (PC), Italy

3 Departamento de Mejora Genética, IAS-CSIC, Apdo. 4084, Cordoba 14980, Spain

4 Center for Genome Research, Biomedical Sciences Department, Biological Chemistry Section, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy

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BMC Genomics 2013, 14:868  doi:10.1186/1471-2164-14-868

Published: 10 December 2013

Additional files

Additional file 1: Table S1:

Probe sets detecting transcripts more than two-fold up-or down-regulated in the comparisons between each alloplasmic line and the corresponding euplasmic control.

Format: XLSX Size: 116KB Download file

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Additional file 2: Table S2:

Genes selected for array validation and the corresponding primers employed in the qRT-PCR analysis. Expression data detected with microarray and qRT-PCR analysis are reported for comparison.

Format: XLSX Size: 24KB Download file

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Additional file 3: Table S3:

Data of two-way ANOVA metabolites that significantly changed in the alloplasmic line TH237 (H. chilense cytoplasm) and its euplasmic control T20 due to genotype, light treatment and the interaction effect between them.

Format: XLSX Size: 15KB Download file

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Additional file 4: Table S4:

Data of two-way ANOVA metabolites that significantly changed in the alloplasmic lines T183 (Ae. uniaristata cytoplasm) and T195 (Ae. tauschii cytoplasm) and their euplasmic control (Chris) due to genotype, light treatment and the interaction effect between them.

Format: XLSX Size: 16KB Download file

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Additional file 5: Table S5:

Metabolite variations in the comparison between the alloplasmic line with the H. chilense cytoplasm (TH237) and the corresponding wheat euplasmic control (T20). Values expressed as fold change for plants grown at low light and high light.

Format: XLSX Size: 13KB Download file

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Additional file 6: Table S6:

Metabolite variations in the comparison between the alloplasmic lines with Ae. uniaristata (T183) or Ae. tauschii (T195) and the corresponding wheat euplasmic control (Chris). Values expressed as fold change for plants grown at low light and high light.

Format: XLSX Size: 15KB Download file

Open Data

Additional file 7: Table S7:

Fatty acids measured in the alloplasmic line TH237 (H. chilense cytoplasm) and its euplasmic control T20. Data was subjected to a student t-test to test for fatty acids that were significant (p ≤ 0.05) between the alloplasmic line and its euplasmic control.

Format: XLSX Size: 11KB Download file

Open Data