Strategy used in the wheat RNA-Seq analysis. A set of 29,617 wheat transcripts was constructed from 15,047 non-redundant contigs and 14,570 full-length cDNAs in TriFLDB. To identify the responsive transcripts under –P conditions, all reads were aligned to the transcripts using Bowtie, and a G-test with an FDR value cut-off < 0.01 was used.
Oono et al. BMC Genomics 2013 14:77 doi:10.1186/1471-2164-14-77