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Open Access Highly Accessed Research article

Global transcriptome analysis of AtPAP2 - overexpressing Arabidopsisthaliana with elevated ATP

Feng Sun1, Chao Liang1, James Whelan23, Jun Yang4, Peng Zhang4 and Boon Leong Lim15*

  • * Corresponding author: Boon L Lim bllim@hku.hk

  • † Equal contributors

Author Affiliations

1 School of Biological Sciences, the University of Hong Kong, Pokfulam, Hong Kong, China

2 Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA 6009, Australia

3 Botany Department, School of Life Science, La Trobe University, Bundoora 3086 Victoria, Australia

4 National Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China

5 Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China

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BMC Genomics 2013, 14:752  doi:10.1186/1471-2164-14-752

Published: 1 November 2013

Abstract

Background

AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes.

Results

ATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription.

Conclusions

AtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.

Keywords:
Chloroplast; Mitochondria; LHC; Redox; Photosystem; Transcriptomes