Determination of percentage GC bias in the Illumina and PacBio datasets. Percentage of mean depth of coverage across 987 windows of 157 nucleotides plotted as a function of percentage GC content for (a) Illumina (black) and (b) PacBio (green) data showing a much stronger positive dependency within the Illumina data (Pearsons correlation coefficient = 0.61 p-value = 2.2e-16) than in the PacBio data (Pearsons correlation coefficient = 0.23 p-value = 5.675e-09). For the purposes of the calculation, high coverage data from the two inverted repeat regions were excluded.
Ferrarini et al. BMC Genomics 2013 14:670 doi:10.1186/1471-2164-14-670