Figure 6.

Activation of IRS-1 and ERK-1 is under control of miR-296-3p and miR-298-5p in αTC1-6. (A) Western analysis of phospho-IRS-1 (Tyr612) in (1) untreated αTC1-6 transfected for 24 h with (i) scramble molecules (NC); (ii) mimics of miR-296-3p; (iii) mimics of miR-298-5p; (iv) mimics of both miR-296-3p and miR-298-5p (left); (2) αTC1-6 transfected for 24 h with (i) scramble molecules (NC); (ii) mimics of miR-296-3p; (iii) mimics of miR-298-5p; (iv) mimics of both miR-296-3p and miR-298-5p and treated with cytokines for further 24 h (right). (B) Western analysis of phospho-ERK-1/2 (Thr202/Tyr204) performed in the same experimental conditions as in (A). Quantification of immunoblot signals was made by equalizing phospho-specific IRS-1 or Erk1/2 band intensities to total IRS-1 or Erk1/2, respectively. The decrease in phosphorylation was normalized to the basal level of the control and reported in arbitrary units as fold decrease over basal value. Numbers below Actin blots represent fold change expression values relative to matched controls.

Barbagallo et al. BMC Genomics 2013 14:62   doi:10.1186/1471-2164-14-62
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