Additional file 5.
Figure S3.TcABC expression in intestinal/excretory tissues and in carcass. Carcass (CAR) was obtained by removing the head, the two last abdominal segments and the complete gut with adherent Malpighian tubules from a penultimate larva. Total RNA was isolated from pools of carcass and intestinal/excretory tissues (IET) and transcribed into cDNA. Triplicates of independent cDNA preparations were used as templates in qPCRs using primers specific to the respective gene. Mean normalized expression was determined by comparing CT values of the respective target gene and the reference gene TcRPS6. Error bars indicate standard errors. Panel (A) shows TcABC genes with comparably low transcript levels, while panel (B) shows TcABC genes with higher transcript levels.
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Broehan et al. BMC Genomics 2013 14:6 doi:10.1186/1471-2164-14-6