Expression profiles for TcABC genes at different developmental stages as determined by qPCR. Total RNA was isolated from pools of eggs, young larvae, late larvae, pre-pupae, pupae and adults and transcribed into cDNA. Triplicates of independent cDNA preparations were used as templates in qPCRs with primers specific to the respective genes. Mean normalized expression was determined by comparing CT values of the respective target gene and the reference gene TcRPS6. Standard errors are given in the tables below. Panel (A) shows the expression profiles for TcABC genes with comparably low transcript levels, whereas panel (B) shows that for TcABC genes with higher transcript levels.
Broehan et al. BMC Genomics 2013 14:6 doi:10.1186/1471-2164-14-6