Additional file 24: File S18.

Protocol for preparing samples for sequencing of mRNA.

Scripts: Script 1: Perl script for performing blast search. Script 2: Perl script for pre-clustering the blast parsed file. Script 3: Perl script to calculate RPKM for the assembled file. Script S1: Perl script to shuffle short read sequences. Script S2: Perl script to measure the N50 statistics. Script S3: Unix shell script to remove Fasta files shorter than a threshold. Script S4: Generate the sub-Fasta file. Script S5: Extract the cDNA sequences corresponding to ORF files.

Format: PDF Size: 137KB Download file

This file can be viewed with: Adobe Acrobat Reader

Pooyaei Mehr et al. BMC Genomics 2013 14:546   doi:10.1186/1471-2164-14-546