Schema of ditag library construction and data analysis. A) The blue spots represent restriction sites/ends, and the blue arrows represent the SOLiD mate-paired reads, which can be translated into experimental ditags. B) Ditags were identified and sorted as reference-type or variant-type based on the alignments. Then, an in silico PCR program was used to check the alignment’s uniqueness and the accuracy of the ditags for inferring possible deletions.
Gong et al. BMC Genomics 2013 14:51 doi:10.1186/1471-2164-14-51