Additional file 1: Figure S1.

Phylogenetic tree of DBM, Bombyx mori and other insects P450 genes. The amino acid sequences (232 aa or longer) of p450 domain (Pfam ID: PF00067) were extracted (61 domains in DBM; 79 domains in B. mori; 1 domain (CYP321A1) in Helicoverpa zea; 1 domain (CYP6BD6) in Manduca sexta; 1 domain (CYP347A1) in Tribolium castaenum; 1 domain (CYP304F2) in Zygaena filipendulae) and aligned using ClustalW. Phylogenetic tree was built using neighbor-joining method in MEGA5 with a bootstrap of 1,000 replicates, Poisson model for amino acid substitution, and pairwise deletion of gaps. Bootstrap values resulting from 1,000 replicates are shown at the branch points. Each DBM gene ID (PxCYP-XXXXXX) corresponds to PXGS_V2_XXXXXX in KONAGAbase.

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Jouraku et al. BMC Genomics 2013 14:464   doi:10.1186/1471-2164-14-464