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Open Access Highly Accessed Research article

A high-resolution cucumber cytogenetic map integrated with the genome assembly

Jianying Sun1, Zhonghua Zhang2, Xu Zong1, Sanwen Huang2, Zongyun Li13* and Yonghua Han13*

Author Affiliations

1 Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China

2 Key Laboratory of Horticultural Crops Genetic Improvement of Ministry of Agriculture and Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

3 The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, China

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BMC Genomics 2013, 14:461  doi:10.1186/1471-2164-14-461

Published: 9 July 2013

Abstract

Background

High-resolution cytogenetic map can provide not only important biological information on genome organization but also solid foundation for genetic and genomic research. The progress in the molecular and cytogenetic studies has created the basis for developing the cytogenetic map in cucumber (Cucumis sativus L.).

Results

Here, the cytogenetic maps of four cucumber chromosomes (chromosomes 1, 3–5) were constructed by fluorescence in situ hybridization (FISH) analysis on cucumber pachytene chromosomes. Together with our previously constructed cytogenetic maps of three cucumber chromosomes (chromosomes 2, 6–7), cucumber has a complete cytogenetic map with 76 anchoring points between the genetic, the cytogenetic and the draft genome assembly maps. To compare our pachytene FISH map directly to the genetic linkage and draft genome assembly maps, we used a standardized map unit—relative map position (RMP) to produce the comparative map alignments. The alignments allowed a global view of the relationship of genetic and physical distances along each cucumber chromosome, and accuracy and coverage of the draft genome assembly map.

Conclusions

We demonstrated a good correlation between positions of the markers in the linkage and physical maps, and essentially complete coverage of chromosome arms by the draft genome assembly. Our study not only provides essential information for the improvement of sequence assembly but also offers molecular tools for cucumber genomics research, comparative genomics and evolutionary study.