Saturation analysis. ER: (a) and (c); LL: (b) and (d). Saturation analysis of DGE libraries showed that new emerging genes and unique tags were gradually reduced with increasing total sequence tags when the library of total sequencing tags was large enough. Percentage of genes (a and b) or unique tags (c and d) identified gradually decreased with increasing total tags when the number of tag sequenced was high enough.
Zhang et al. BMC Genomics 2013 14:45 doi:10.1186/1471-2164-14-45