Expression of dADA2b isoforms in transgene-carrier dAda2b larvae and rescue ability of dAda2b transgenes. A: dADA2b protein expression in wild type (w1118), dAda2bd842 null mutant and dAda2b transgene-carrier dAda2bd842 animals. The genotypes are indicated on top. Transgenes carrying EGFP-fused dADA2b isoform were expressed from the native dAda2b promoter. Protein extracts of third instar larvae and pharate adults were separated on SDS-PAGE and immunoblot was prepared using dADA2b specific polyclonal antibody. The positions of native dADA2bS and dADA2bL isoforms are indicated on the left, that of the EGFP tagged forms of the isoforms are shown on the right. Note that the native dADA2bS isoform is also produced in dAda2bL-EGFP transgene-carriers, due to alternative splicing of the mRNA. B: Rescue of dAda2bd842 mutants by transgenes expressing dADA2b isoforms. Transgenes carrying EGFP-fused dADA2b isoform were expressed from the native dAda2b promoter, while the expression of UAS-dAda2bS transgene was driven by da-GAL4 driver, as indicated. The graph shows the percentage of larvae eclosed as adults in each genotype tested.
Pankotai et al. BMC Genomics 2013 14:44 doi:10.1186/1471-2164-14-44