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Open Access Highly Accessed Research article

Genome-wide identification and expression analysis of calcium-dependent protein kinase in maize

Xiangpei Kong, Wei Lv, Shanshan Jiang, Dan Zhang, Guohua Cai, Jiaowen Pan and Dequan Li*

Author Affiliations

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong, 271018, China

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BMC Genomics 2013, 14:433  doi:10.1186/1471-2164-14-433

Published: 1 July 2013

Additional files

Additional file 1: Figure S1:

Subcellular localization of the ZmCPK5:GFP fusion protein in onion epidermal cells. The cells with constructs expressing GFP alone and the ZmCPK5:GFP fusion protein were analyzed under bright and fluorescence field after 16 h.

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Additional file 2: Figure S2:

Exon–intron structures of maize-rice orthologs genes. Green boxes, exons; lines, introns; Blue boxes, UTR; 0,1,2, intron phase.

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Additional file 3: Figure S3:

Phylogenetic tree of maize CDPKs. Neighbor-joining tree was created using MEGA5.0 program with 1,000 bootstrap using full length sequences of 40 maize. Four groups were labeled as I, II, III, and IV.

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Additional file 4: Table S1:

PCR primers used in this study.

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