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Open Access Research article

Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly

Chitvan Khajuria15, Haiyan Wang2, Xuming Liu1, Shanda Wheeler1, John C Reese1, Mustapha El Bouhssini3, R Jeff Whitworth1 and Ming-Shun Chen14*

Author Affiliations

1 Department of Entomology, Kansas State University, Manhattan 66506, Kansas

2 Department of Statistics, Kansas State University, Manhattan 66506, Kansas

3 International Center for Agricultural Research in the Dry Area, Allepo, Syria

4 Hard Winter Wheat Genetics Research Unit, USDA-ARS, Kansas State University, 4008 Throckmorton, Manhattan 66506, Kansas

5 Current Address: Department of Entomology, University of Nebraska, Lincoln 68583, Nebraska

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BMC Genomics 2013, 14:423  doi:10.1186/1471-2164-14-423

Published: 26 June 2013

Abstract

Background

Wheat – Hessian fly interaction follows a typical gene-for-gene model. Hessian fly larvae die in wheat plants carrying an effective resistance gene, or thrive in susceptible plants that carry no effective resistance gene.

Results

Gene sets affected by Hessian fly attack in resistant plants were found to be very different from those in susceptible plants. Differential expression of gene sets was associated with differential accumulation of intermediates in defense pathways. Our results indicated that resources were rapidly mobilized in resistant plants for defense, including extensive membrane remodeling and release of lipids, sugar catabolism, and amino acid transport and degradation. These resources were likely rapidly converted into defense molecules such as oxylipins; toxic proteins including cysteine proteases, inhibitors of digestive enzymes, and lectins; phenolics; and cell wall components. However, toxicity alone does not cause immediate lethality to Hessian fly larvae. Toxic defenses might slow down Hessian fly development and therefore give plants more time for other types of defense to become effective.

Conclusion

Our gene expression and metabolic profiling results suggested that remodeling and fortification of cell wall and cuticle by increased deposition of phenolics and enhanced cross-linking were likely to be crucial for insect mortality by depriving Hessian fly larvae of nutrients from host cells. The identification of a large number of genes that were differentially expressed at different time points during compatible and incompatible interactions also provided a foundation for further research on the molecular pathways that lead to wheat resistance and susceptibility to Hessian fly infestation.