Open Access Research article

Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis

Jiajie Sun1, Mijie Li1, Zhuanjian Li1, Jing Xue1, Xianyong Lan1, Chunlei Zhang2, Chuzhao Lei1 and Hong Chen1*

Author Affiliations

1 College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China

2 Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China

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BMC Genomics 2013, 14:42  doi:10.1186/1471-2164-14-42

Published: 18 January 2013

Additional files

Additional file 1:

Bovine conserved miRNAs.

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Additional file 2:

The annotated miRNA and its miRNA* in the fetal bovine library.

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Additional file 3:

The known miRNAs expression profiles between two libraries.

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Additional file 4:

Nucleotide bias at each position of sRNA tags. Note: miRNA nucleotide bias at each position of adult bovine muscle tissue (A) and fetal bovine muscle tissue (B).

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Additional file 5:

Summary of base edit in adult bovine library.

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Additional file 6:

Summary of base edit in fetal bovine library.

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Additional file 7:

End variants of the fetal bovine library identified in this study.

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Additional file 8:

Family analyses of known miRNAs.

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Additional file 9:

Novel miRNAs identified in this study.

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Additional file 10:

The expression of miRNAs in bovine tissues and organs were detected by RT-qPCR. Note: FM: fetal bovine muscle tissue; CM: calf bovine muscle tissue; AM: adult bovine muscle tissue.

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Additional file 11:

Stem-loop RT-PCR Primer.

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