Additional file 8.
Inhibition of the MAPK pathway in HeLa cells. HeLa cells were serum-starved for 24 hours and treated with EGF for 6 hours in the presence or absence of protein kinase inhibitors: AG1470 (EGFR inhibitor), U0126 (MEK inhibitor) and Wortmannin (PI3K inhibitor). In addition, HeLa cells were transfected with a constitutively active form of Ras (RasV12). Lysates were analysed by Western-Blot against pospho-ERK1/2 and phospho-AKT to ensure protein inhibitors and transfection action over MAPK and AKT pathways. Tubulin was used as sample loading control.
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Llorens et al. BMC Genomics 2013 14:371 doi:10.1186/1471-2164-14-371