Open Access Highly Accessed Research article

Sleep is not just for the brain: transcriptional responses to sleep in peripheral tissues

Ron C Anafi1*, Renata Pellegrino123, Keith R Shockley4, Micah Romer1, Sergio Tufik3 and Allan I Pack1

Author Affiliations

1 Division of Sleep Medicine and Center for Sleep and Circadian Neurobiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

2 Center for Applied Genomics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA

3 Universidade Federal de São Paulo UNIFESP, São Paulo, Brazil

4 Department of Health and Human Services, Biostatistics Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA

For all author emails, please log on.

BMC Genomics 2013, 14:362  doi:10.1186/1471-2164-14-362

Published: 30 May 2013

Additional files

Additional file 1:

Comma separated value (CSV) file listing sleep enhanced and sleep repressed genes in heart and lung.

Format: CSV Size: 12KB Download file

Open Data

Additional file 2: Figure S1:

KEGG Pathway diagram depicting ubiquitin mediated proteolysis. Transcripts enhanced by sleep in the heart are colored light blue. Transcripts enhanced by sleep in both heart and lungs are colored dark blue.

Format: PNG Size: 34KB Download file

Open Data

Additional file 3: Figure S2:

Effect of sleep and sleep deprivation on the inter-animal variability of transcript expression in the lung as assessed by median deviations. 210 probes showed statistically significant changes in expression variability as a function of sleep duration. The median absolute deviation (MAD) is divided by median expression to obtain a normalized measure of variation resistant to outliers. The normalized MAD among spontaneously sleeping mice after 3, 6, 9 and 12 hours is plotted against the normalized MAD of the same transcript at baseline (lights-on). The red line of identity (x = y) demarcates no change in inter-animal variability. Transcripts with statistically significant temporal changes in variance among sleeping mice were colored in red. As shown in Figure 6A, these transcripts remain clustered and show systematic changes with variability as sleep progresses.

Format: PDF Size: 808KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data