Open Access Research article

Escherichia coli- and Staphylococcus aureus-induced mastitis differentially modulate transcriptional responses in neighbouring uninfected bovine mammary gland quarters

Kirsty Jensen1*, Juliane Günther2, Richard Talbot3, Wolfram Petzl4, Holm Zerbe4, Hans-Joachim Schuberth5, Hans-Martin Seyfert2 and Elizabeth J Glass1

Author Affiliations

1 Division of Infection & Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK

2 Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany

3 ARK-Genomics Facility, The Roslin Institute and R(D)SVS, Easter Bush, Midlothian, Edinburgh, EH25 9RG, UK

4 Clinic for Ruminants, Ludwig-Maximilians-University, Munich, Germany

5 Institute of Immunology, University of Veterinary Medicine, Hannover, Germany

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BMC Genomics 2013, 14:36  doi:10.1186/1471-2164-14-36

Published: 16 January 2013

Additional files

Additional file 1:

List of clones exhibiting statistically significant differential expression between control quarters from E. coli and S. aureus infected cows. An excel file listing the 255 clones, representing 187 known genes, exhibiting differential expression between control quarters from E. coli and S. aureus infected cows (fold difference ≥ 2, FDR < 0.05). The fold differences between the control quarters from E. coli (EC24T0) and S. aureus (SA24T0) infected cattle are displayed as a positive number when EC24T0>SA24T0 (highlighted in orange) and a negative number when SA24T0>EC24T0 (highlighted in green). Genes shown in bold are represented by more than one clone. The calculated FDR values are included.

Format: XLS Size: 66KB Download file

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Additional file 2:

List of clones exhibiting statistically significant differential expression between control quarters from cows infected with S. aureus for 24 and 72 hours. An excel file listing the 115 clones, representing 76 known genes, exhibiting differential expression between control quarters from cows infected with S. aureus for 24 (SA24T0) and 72 hours (SA72T0) (fold difference ≥ 2, P < 0.01). The fold differences between the control quarters from animals infected with S. aureus for 24 hours (SA24T0) or 72 hours (SA72T0) are displayed as a positive number when SA24T0>SA72T0 (highlighted in orange) and a negative number when SA72T0>SA24T0 (highlighted in green). Genes shown in bold are represented by more than one clone. The calculated P values are included.

Format: XLS Size: 41KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Infection of neighbouring quarters affects the levels of TGFB1 mRNA in uninfected quarters. Comparison of TGFB1 mRNA levels in uninfected quarters of cattle infected for 24 hours with S. aureus (SA24T0), E. coli (EC24T0) or from healthy, uninfected (HEALTHY) cattle. TGFB1 levels are expressed as relative copy numbers and the bars denote standard error of the mean. The statistical significance is indicated as P values and n.s. denotes not significant. The HEALTHY samples were included in a previously published study [17]. Animals in both studies were selected using the same criteria and hormonally synchronized using the same procedure.

Format: PPTX Size: 108KB Download file

Open Data