Characterization of human plasma-derived exosomal RNAs by deep sequencing
1 Department of Pathology and Cancer Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA
2 Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA
3 Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, 55905, USA
4 Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China
5 Department of Physiology, Medical College of Wisconsi, Milwaukee, WI, 53226, USA
6 Department of Oncology, Mayo Clinic, Rochester, MN, 55905, USA
7 Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA
BMC Genomics 2013, 14:319 doi:10.1186/1471-2164-14-319Published: 10 May 2013
Additional file 1:
Percentage of read counts with different insert sizes among the total mappable reads. The NEBNext multiplex small RNA library preparation kit (NEB) generated more sequences with 21–23 nt inserts than did the other two kits that were tested. Overall represents the averages of the three different kits that were tested.
Format: TIFF Size: 1.5MB Download file
Additional file 2:
Read counts of the miRNAs detected in the 14 libraries (normalized to read number per million mappable miRNA seqeuences).
Format: XLSX Size: 62KB Download file
Additional file 3:
Top 20 RNAs in other RNA species (normalized to read number per million all mappable RNA seqeuences).
Format: XLSX Size: 28KB Download file
Additional file 4:
Putative miRNAs predicted by miRDeep2.
Format: XLSX Size: 24KB Download file