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Open Access Highly Accessed Research article

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04

Joakim M Andersen12, Rodolphe Barrangou3, Maher Abou Hachem1, Sampo J Lahtinen4, Yong Jun Goh2, Birte Svensson1 and Todd R Klaenhammer2*

Author Affiliations

1 Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Søltofts Plads Building 224, Kgs. Lyngby DK-2800, Denmark

2 Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh NC 27695, USA

3 DuPont Nutrition and Health, 3329 Agriculture Drive, Madison WI 53716, USA

4 DuPont Nutrition and Health, Sokeritehtaantie 20, Kantvik FI-02460, Finland

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BMC Genomics 2013, 14:312  doi:10.1186/1471-2164-14-312

Published: 10 May 2013

Abstract

Background

Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of α- and β-linked hexoses, and β-xylosides.

Results

The overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and β-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and β-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria.

Conclusion

This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.

Keywords:
Bifidobacterium lactis; Transcriptomics; ABC transporter; GPH transporter; Prebiotics; Glycoside hydrolase