Open Access Research article

Transcriptional pathways associated with the slow growth phenotype of transformed Anaplasma marginale

Sebastián Aguilar Pierlé*, Gena Kenitra Hammac, Guy H Palmer and Kelly A Brayton*

Author Affiliations

Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, 99164-7040, USA

For all author emails, please log on.

BMC Genomics 2013, 14:272  doi:10.1186/1471-2164-14-272

Published: 22 April 2013

Additional files

Additional file 1:

Whole genome transcriptional profiling of AmTR versus wild typeA. marginale. The genome wide transcriptional comparison of wild type and AmTR across three biological replicates is reported. Fold changes, total gene reads, raw and normalized expression values, p-values for Baggerly’s test and Cluster affiliation for each feature are shown.

Format: XLSX Size: 127KB Download file

Open Data

Additional file 2:

Validation of the transcriptional status of 6 genes through qPCR. The fold changes of 6 genes found through RNA-seq (blue) and qPCR (red), across two biological replicates are reported.

Format: PPTX Size: 43KB Download file

Open Data

Additional file 3:

Fold change found for genes involved in translation pathway. The fold changes found for genes involved in translation pathway in AmTR compared to wild type are reported. P values smaller than 1E-16 are reported as 0.

Format: DOCX Size: 18KB Download file

Open Data

Additional file 4:

GSEA on whole genome transcriptional profiling of wild typeA. marginale and AmTR. The different pathways evaluated in through GSEA are shown. Lower and Upper tail values show the mass in the permutation based p-value distribution below or above the value of the test statistic.

Format: XLSX Size: 20KB Download file

Open Data