Table 1

Genes of the Plasmodium falciparum gametocyte transcriptome as identified by SSH (comparing mature non-activated gametocytes and gametocytes at 30 min p.a.)
Plasmodb ID Protein Predicted features pred. MW
Signaling
PF3D7_0214600 serine/threonine protein kinase 204.3
PF3D7_1356800 serine/threonine protein kinase ARK3 475.7
PF3D7_0302100 serine/threonine protein kinase PfCLK-4/SRPK1 Phosphorylation of SR splicing factors 157.4
PF3D7_1230900 atypical protein kinase, RIO family 111.5
PF3D7_1238900 calcium/calmodulin-dependent protein kinase, PK2 58.9
PF3D7_0624000 hexokinase, HK 55.3
PF3D7_0515300 phosphatidylinositol 3-kinase, PI3K 255.9
PF3D7_1113100 protein tyrosine phosphatase, PRL 25.1
PF3D7_1403900 phosphatase 35.2
PF3D7_1464600 phosphatase SP 170.5
PF3D7_0210600 conserved Plasmodium protein SP, calcium-lipid-binding domain CaLB 53.7
PF3D7_1238100 calcyclin binding protein Calcyclin-binding domain 266.4
PF3D7_1442200 GTP binding protein 118.7
PF3D7_1358600 zinc finger protein Zinc-Finger domain 118.3
PF3D7_0927200 zinc finger protein Zinc-Finger domain 189.7
PF3D7_1004300 zinc finger, C3HC4 type 131.7
PF3D7_1433400 conserved Plasmodium membrane protein 7 TMs, PHD C4HC3 zinc-finger-like motifs 682.5
PF3D7_1463900 conserved Plasmodium membrane protein SP, 7 TMs, EF hand calcium-binding domain 127.1
PF3D7_1233600 asparagine and aspartate rich protein 1, AARP1 Zinc-finger domain, Ring/U-Box 146.3
PF3D7_0530200 triose phosphate transporter iTPT SP, 7 TMs 59.8
PF3D7_0303900 conserved Plasmodium protein SP, phosphatidyl-ethanolamine-binding protein PEBP domain 23.2
PF3D7_0818200 14-3-3 protein Regulative protein, binds signaling enzymes like kinases or TM receptors 30.2
Cell cycle and gene expression
PF3D7_1445900 DEAD/DEAH box ATP-dependent RNA helicase 60.0
PF3D7_1019000 eukaryotic translation initiation factor subunit eIF2A 280.8
PF3D7_1417200 NOT family protein Regulation of transcript 519.8
PF3D7_1103800 CCR4-NOT transcription complex subunit 1, NOT1 4 TMs 392.0
PF3D7_0622900 ApiAP2 protein Transcription factor 237.4
PF3D7_0420300 ApiAP2 protein Transcription factor 400.2
PF3D7_0615400 ribonuclease 323.2
PF3D7_0811300 CCR4-associated factor 1, CAF1 200.2
PF3D7_1034900 methionine-tRNA ligase 101.2
PF3D7_1469700 conserved Plasmodium protein Homology to the transcriptional mediator subunit Med6 24.6
PF3D7_0513600 deoxyribodipyrimidine photolyase 129.2
PF3D7_1105500 centrin-4 (CEN4) 20.1
PF3D7_1018500 PHF5-like protein mRNA splicing factor RDS3 domain 12.5
PF3D7_0827800 SET domain protein SET3 Chromatin regulator, nuclear protein, in male gametocytes 283.6
PF3D7_1115200 SET domain protein, SET7 Chromatin regulator 94.3
PF3D7_1360900 polyadenylate-binding protein RNA-binding domain 44.8
PF3D7_1011800 QF122 antigen KH domain type 1, RNA binding 131.6
PF3D7_1347500 DNA/RNA-binding protein Alba 4, ALBA4 42.2
Cytoskeleton, IMC, Motor complex
PF3D7_0422300 alpha-tubulin II Cytoskeletal element 49.7
PF3D7_1246200 actin I IMC component 41.2
PF3D7_0503400 actin-depolymerizing factor 1, ADF1 13.7
PF3D7_1020200 kinesin 99.1
PF3D7_0724900 kinesin-like protein 222.6
PF3D7_1246400 myosin A tail domain interacting protein, MTIP IMC component 23.5
PF3D7_1251200 coronin Actin-binding protein 69.0
PF3D7_0708000 cytoskeleton-associated protein Cap-Gly domain 103.6
PF3D7_0515700 glideosome-associated protein 40, GAP40 10 TMs, IMC component 51.8
PF3D7_0918000 glideosome-associated protein 50, GAP50 SP, 2 TMs, IMC component 44.6
PF3D7_1351700 Alveolin, ALV6 IMC component 151.2
Proteostasis (Protein trafficking, stabilization and degradation)
PF3D7_0807500 proteasome subunit alpha type 6 Component oft he proteasome core particle 29.5
PF3D7_1466300 proteasome subunit RPN2 Proteasome non-ATPase regulatory subunit 1 132.8
PF3D7_0815800 vacuolar sorting protein, VPS9 Ortholog of Saccharomyces cerevisae vacuolar sorting protein 9 214.7
PF3D7_1136400 conserved Plasmodium protein TPR domain, mediates protein interactions and multi-protein complexes 107.1
PF3D7_0215400 conserved Plasmodium protein WD40-domain protein, assembly and stabilization of MPCs 110.8
PF3D7_0818900 heat shock protein 70, HSP70-1 73.9
PF3D7_0917900 heat shock protein 70, HSP70-2 72.4
PF3D7_0306200 activator of Hsp90 ATPase, AHA1 41.6
PF3D7_0708400 heat shock protein 90, HSP90 86.2
PF3D7_0102200 ring-infected erythrocyte surface antigen, RESA PHIST and DnaJ domains 126.5
Metabolism, general
mal_mito_2 cytochrome c oxidase subunit I, cox1 SP, 12 TMs, component of the respiratory chain in mitochondria 53.0
PF3D7_1444800 fructose 1,6-biphosphate aldolase Glycolysis pathway 40.1
PF3D7_0806800 vacuolar proton translocating ATPase subunit A 6 TMs 123.0
PF3D7_0922200 S-adenosylmethionine synthetase, SAMS 44.8
PF3D7_0801900 conserved Plasmodium protein FAD/NADP-binding domain 552.0
PF3D7_0403200 conserved Plasmodium protein Possibly acetyl-CoA-synthetase 69.4
PF3D7_0511800 myo-inositol 1-phosphate synthase 69.1
PF3D7_0104400 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, LytB 1 TM, protein export domain, non-mevalonate pathway 62.5
cell surface and PVM
PF3D7_1035300 glutamate-rich protein; GLURP SP, on parasite surface 141.1
PF3D7_1335300 reticulocyte binding protein 2, RH2b 1 TM, on parasite surface, involved in erythrocyte binding 382.9
PF3D7_1228600 merozoite Surface Protein 9, MSP-9 SP, on merozoite surface, involved in erythrocyte binding 86.6
PF3D7_1028700 merozoite TRAP-like protein, MTRAP SP, 1 TM, belongs to TRAP family, in micronemes and on merozoite surface 58.1
PF3D7_0731500 erythrocyte binding antigen 175, EBA-175 SP, 1 TM, Duffy binding domain, on merozoite surface 174.6
PF3D7_0102500 erythrocyte binding antigen-181, EBA-181 SP, 2 TMs, Duffy binding domain, on merozoite surface 181.2
PF3D7_1035700 duffy binding-like merozoite surface protein, DBLMSP SP, Duffy binding domain, SPAM 80.3
PF3D7_0202500 early transcribed membrane protein 2, ETRAMP2 SP, 1 TM, integral PVM protein 11.5
PF3D7_0423700 early transcribed membrane protein 4, ETRAMP4 SP, 2TMs, integral PVM protein 10.2
PF3D7_1033200 early transcribed membrane protein 10.2, ETRAMP 10.2 SP, 2TMs, integral PVM protein 38.9
PF3D7_1103500 CPW-WPC family protein SP, adhesin, cysteine-rich CPW-WPC domain 67.3
PF3D7_0406200 Pfs16 SP, 2 TMs, integral PVM protein of gametocytes 16.7
PF3D7_0930300 merozoite surface protein 1, MSP1 SP, GPI anchor, EGF domain, on merozoite surface, part of MSP complex 195.7
PF3D7_1218800 conserved Plasmodium protein, PSOP17 SP, lectin domain, laminin G2 domain 39.6
PF3D7_0707300 rhoptry-associated membrane antigen, RAMA GPI anchor, involved in erythrocyte binding 103.6
PF3D7_1021900 10b antigen Ankyrin repeat region, PHAX RNA-binding domain, interacts with surface proteins like PfEMP1 (Y2H) 267.4
Others
PF3D7_1103100 60S ribosomal protein P1 13.0
PF3D7_1027800 60S ribosomal protein L3 44.2
PF3D7_1408600 40S ribosomal protein S8e 25.1
PF3D7_1358800 40S ribosomal protein S13 17.3
PF3D7_0206200 metabolite/drug transporter 11 TMs, Major facilitator superfamily 62.7
PF3D7_1207700 blood stage antigen 41–3 precursor SP 43.4
PF3D7_1457000 signal peptide peptidase, SPP 8 TMs 47.6
PF3D7_0320700 signal peptidase complex subunit 2, SPC2 2 TMs, SPC25 domain 21.0
PF3D7_0207700 serine repeat antigen 4, SERA-4 SP, papain family cysteine proteinase 108.7
PF3D7_1033800 plasmepsin VII SP, 1 TM, aspartyl protease 52.3
PF3D7_0930000 procollagen lysine 5-dioxygenase 2 TMs 66.8
PF3D7_0817600 conserved Plasmodium protein MAC/PF domain 112.2
PF3D7_0403800 conserved Plasmodium protein Alpha/beta-hydrolase 83.4
PF3D7_0709900 conserved Plasmodium membrane protein 4 TMs, alpha/beta-hydrolase 274.0
PF3D7_0311600 conserved Plasmodium protein, unknown function SP, 1 TM, ribophorin-I domain,putative dolichyl-diphospho-oligosaccharide-protein 84.0
Unknown
PF3D7_0904200 conserved Plasmodium protein SP, 1 TM 34.0
PF3D7_1024800 conserved Plasmodium protein SP, 2 TMs 171.1
PF3D7_1239400 conserved Plasmodium protein SP 22.0
PF3D7_0730800.1 Plasmodium exported protein SP 32.1
PF3D7_1316700 conserved Plasmodium protein SP 73.4
PF3D7_0601900 conserved Plasmodium protein 1 TM 14.7
PF3D7_0704100 conserved Plasmodium membrane protein 6 TMs 425.3
PF3D7_1023000 conserved Plasmodium membrane protein 5 TMs 48.9
PF3D7_1439600 conserved Plasmodium protein 1 TM 22.6
PF3D7_1021700 conserved Plasmodium membrane protein 4 TMs 830.2
PF3D7_0417400 conserved Plasmodium protein 2 TMs, interacts with PF3D7_1021700 (Y2H) 822.4
PF3D7_1026600 conserved Plasmodium protein Interacts with RNA-associated proteins (Y2H) 196.5
PF3D7_1362600 conserved Plasmodium protein Interacts with splicing factor 3B subunit 2 (Y2H) 32.1
PF3D7_0202400 conserved Plasmodium protein Pfg27 domain 141.9
PF3D7_1124200 conserved Plasmodium protein UAS domain 49.3
PF3D7_1452400 conserved Plasmodium protein 107.5
PF3D7_1225600 conserved Plasmodium protein 93.1
PF3D7_1451200 conserved Plasmodium protein 176.2
PF3D7_1438800 conserved Plasmodium protein 84.0
PF3D7_1114600 conserved Plasmodium protein 39.6
PF3D7_1026100 conserved Plasmodium protein 23.9
PF3D7_1230100 conserved Plasmodium protein 61.5
PF3D7_1321000 conserved Plasmodium protein 80.6
PF3D7_0411000 conserved Plasmodium protein 179.7
PF3D7_1126700 conserved Plasmodium protein 110.1
PF3D7_0508900 conserved Plasmodium protein 370.2

IMC, inner membrane complex; PVM, parasitophorous vacuole membrane; SP, signal peptide; TM, transmembrane; Y2H, yeast-two-hybrid.

Ngwa et al.

Ngwa et al. BMC Genomics 2013 14:256   doi:10.1186/1471-2164-14-256

Open Data