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Open Access Highly Accessed Research article

Identification of drought-responsive and novel Populus trichocarpa microRNAs by high-throughput sequencing and their targets using degradome analysis

Peng Shuai, Dan Liang, Zhoujia Zhang, Weilun Yin* and Xinli Xia*

Author Affiliations

College of Biological Sciences and Biotechnology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, P.R. China

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BMC Genomics 2013, 14:233  doi:10.1186/1471-2164-14-233

Published: 9 April 2013

Additional files

Additional file 1: S1:

Summary of P. trichocarpa small RNAs sequencing.

Format: XLSX Size: 12KB Download file

Open Data

Additional file 2: S2:

30 undetected miRNAs in this study.

Format: XLSX Size: 11KB Download file

Open Data

Additional file 3: S3:

Potential miRNA candidates without miRNA*s found only in one library of P. trichocarpa.

Format: XLSX Size: 50KB Download file

Open Data

Additional file 4: S4:

The predicted hairpin structures of all the 65 new miRNAs precursors.

Format: PDF Size: 202KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5: S5:

MiRNAs in response to drought stress.

Format: XLSX Size: 12KB Download file

Open Data

Additional file 6: S6:

Targets prediction of new P. trichocarpa miRNAs.

Format: XLSX Size: 43KB Download file

Open Data

Additional file 7: S7:

Summary of P. trichocarpa degradome sequencing.

Format: XLSX Size: 12KB Download file

Open Data

Additional file 8: S9:

Leaf water potential.

Format: XLSX Size: 11KB Download file

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Additional file 9: S10:

Leaf photosynthetic data.

Format: XLSX Size: 13KB Download file

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Additional file 10: S8:

The primers designed for RT-qPCR.

Format: XLSX Size: 11KB Download file

Open Data