Open Access Highly Accessed Research article

CAMTA 1 regulates drought responses in Arabidopsis thaliana

Neha Pandey1, Alok Ranjan2, Poonam Pant1, Rajiv K Tripathi1, Farha Ateek1, Haushilla P Pandey3, Uday V Patre1 and Samir V Sawant1*

Author Affiliations

1 Council of Scientific and Industrial Research-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, INDIA

2 Present address: Centre for AIDS Health Disparities Research, Meharry Medical College, Nashville, TN 37208, USA

3 Department of Biochemistry, Banaras Hindu University, Varanasi, India

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BMC Genomics 2013, 14:216  doi:10.1186/1471-2164-14-216

Published: 2 April 2013

Additional files

Additional file 1:

Detailed information of ATCAMTA1-6 mutant with T-DNA insertion site.

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Additional file 2:

Screening of different CAMTA mutant for drought tolerance. By using different mannitol concentration in MS media by root bending assay to show differential primary root growth of Col-0 and CAMTA mutant. (A) Col-0, (B) camta1, (C) camta2, (D) camta3, (E) camta4, (F) camta5, (G) camta6.

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Additional file 3:

The phenotypic characterisation of camta1 mutant and Col-0 under drought stress and the relative expression of CAMTA1 in mutant and Col-0. Quantitative phenotypic measurements of Col-0, camta1-2 and camta1-3. The 3 weeks old plants were subjected to drought stress and after 14 days of stress the measurements have been recorded. Data are given as averages ± SD for 25 plants.The Expression of CAMTA1 gene in Col-0 and camta1-2 and camta1-3 by RT-PCR.

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Additional file 4:

Gene description of differentially regulated genes in leaf tissue of Col-0. List of up-regulated genes of leaf tissue in WT-D/WT-C_L (Fold change ≥ 2) (work sheet 1). List of down-regulated genes of leaf tissue in WT-D/WT-C_L (Fold change ≥ 2) (work sheet 2).

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Additional file 5:

Gene description of differentially regulated genes in leaf tissue of camta1-3. List of up-regulated genes of leaf tissue in M1-D/M1-C_L (Fold change ≥ 2) (work sheet 1). List of down-regulated genes of leaf tissue in M1-D/M1-C_L (Fold change ≥ 2 ) (work sheet 2).

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Additional file 6:

List of CAMTA1 dependent genes of leaf tissue. List of leaf CAMTA1 dependent positively regulated genes (LCDPRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 1). List of leaf CAMTA1 dependent negatively regulated genes (LCDNRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 2). List of leaf CAMTA1 independent drought induced genes (LCIDIG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 3). List of leaf CAMTA1 independent drought repressed genes (LCIDRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 4).

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Additional file 7:

Gene description of differentially regulated genes in root tissue of Col-0. List of up regulated genes of root tissue in WT-D/WT--_R (Fold change ≥ 2) (work sheet 1). List of down regulated genes of root tissue in WT-D/WT--_R (Fold change ≥ 2) (work sheet 1).

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Additional file 8:

Gene description of differentially regulated genes in root tissue of camta1-3. List of up-regulated genes of root tissue in M1-D/M1-C-R (Fold change ≥ 2) (work sheet 1). List of down-regulated genes of root tissue in M1-D/M1-_R (Fold change ≥ 2) (work sheet 2).

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Additional file 9:

List of CAMTA1 dependent genes of root tissue. List of root CAMTA1 dependent positively regulated genes (RCDPRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 1). List of root CAMTA1 dependent negatively regulated genes (RCDNRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 2). List of root CAMTA1 independent drought induced genes (RCIDIG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 3). List of root CAMTA1 independent drought repressed genes (RCIDRG) sorted in accordance with its 2 recognition motif (MCGTGT; MCGCGB) (work sheet 4).

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Additional file 10:

The comparison between Col-0 and camta1-3 under water condition. (A) The relative expression of CAMTA1 gene in Col-0 under water and drought condition. (B) The gene count of differentially expressed genes (P value ≤ 0.05 and FC ≥ 2) of WT-C/M1-C in leaf and root tissue. (C) the GO annotation of the differentially expressed genes in WT-C/M1-C in leaf and root tissue.

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Additional file 11:

Gene description of abiotic stress related genes in CAMTA1 dependent genes of leaf tissue. List of CAMTA positively regulated genes related to various abiotic stress conditions in leaf tissue (LCDPRG) (work sheet 1). List of CAMTA negatively regulated genes related to various abiotic stress conditions in leaf tissue (LCDNRG) (work sheet 2). Percentage of CAMTA recognition motif (CGCG and CGTG) in various stress conditions (work sheet 3).

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Additional file 12:

Gene description of abiotic stress related genes in CAMTA1 dependent genes of root tissue. List of CAMTA positively regulated genes related to various abiotic stress conditions in root tissue (RCDPRG) (work sheet 1). List of CAMTA negatively regulated genes related to various abiotic stress conditions in root tissue (RCDNRG) (work sheet 2). Percentage of CAMTA recognition motif (CGCG and CGTG) in various stress conditions (work sheet 3).

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Additional file 13:

Gene description of phytohormone associated genes in CAMTA1 dependent genes of leaf tissue. List of phytohormones associated genes positively regulated by CAMTA in leaf tissue (LCDPRG) (work sheet 1). List of phytohormones associated genes negatively regulated by CAMTA in leaf tissue (LCDNRG) (work sheet 2).

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Additional file 14:

Gene description of phytohormone associated genes in CAMTA1 dependent genes of root tissue. List of phytohormones associated genes positively regulated by CAMTA in root tissue (RCDPRG) (work sheet 1). List of phytohormones associated genes negatively regulated by CAMTA in root tissue (RCDNRG) (work sheet 2).

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Additional file 15:

Gene description of transcription factor in CAMTA1 dependent genes of leaf tissue. List of TF positively regulated by CAMTA in leaf tissue (LCDPRG) (work sheet 1). List of TF negatively regulated by CAMTA in leaf tissue (LCDNRG) (work sheet).

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Additional file 16:

Gene description of transcription factor in CAMTA1 dependent genes of root tissue. List of TF positively regulated by CAMTA in root tissue (RCDPRG) (work sheet 1). List of TF negatively regulated by CAMTA in leaf tissue (RCDNRG) (work sheet).

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Additional file 17:

The comparison of transcriptome of camta1-2 and camta1-3. (A) The correlation graph between camta1-2 and camta1-3 gene expression profile from microarray data. The venn analysis shows unique and common significantly expressed genes in both mutants. (B) The Mapman analysis of camta1-3.

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Additional file 18:

Primer sequence of genes used for validation by RT-PCR.

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