Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Database

TRACER: a resource to study the regulatory architecture of the mouse genome

Chao-Kung Chen1, Orsolya Symmons2, Veli Vural Uslu2, Taro Tsujimura2, Sandra Ruf2, Damian Smedley13* and François Spitz2*

Author affiliations

1 European Bioinformatics Institute - European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK

2 Developmental Biology Unit - European Molecular Biology Laboratory, Heidelberg, Germany

3 Present address: Welcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK

For all author emails, please log on.

Citation and License

BMC Genomics 2013, 14:215  doi:10.1186/1471-2164-14-215

Published: 2 April 2013

Abstract

Background

Mammalian genes are regulated through the action of multiple regulatory elements, often distributed across large regions. The mechanisms that control the integration of these diverse inputs into specific gene expression patterns are still poorly understood. New approaches enabling the dissection of these mechanisms in vivo are needed.

Results

Here, we describe TRACER (http://tracerdatabase.embl.de webcite), a resource that centralizes information from a large on-going functional exploration of the mouse genome with different transposon-associated regulatory sensors. Hundreds of insertions have been mapped to specific genomic positions, and their corresponding regulatory potential has been documented by analysis of the expression of the reporter sensor gene in mouse embryos. The data can be easily accessed and provides information on the regulatory activities present in a large number of genomic regions, notably in gene-poor intervals that have been associated with human diseases.

Conclusions

TRACER data enables comparisons with the expression pattern of neighbouring genes, activity of surrounding regulatory elements or with other genomic features, revealing the underlying regulatory architecture of these loci. TRACER mouse lines can also be requested for in vivo transposition and chromosomal engineering, to analyse further regions of interest.

Keywords:
Gene regulation and expression; Genome organisation; Regulatory landscapes; Chromosomal engineering; Mouse models of human structural variation